原发性纤维肌痛中的一氧化氮、脂质过氧化产物和抗氧化剂及其与疾病严重程度的相关性。
Nitric oxide, lipid peroxidation products, and antioxidants in primary fibromyalgia and correlation with disease severity.
作者信息
Shukla Varsha, Kumar Das Siddharth, Ali Mahdi Abbas, Agarwal Shweta, Khandpur Sukhanshi
机构信息
King George's Medical University, Department of Rheumatology, Lucknow, Uttar Pradesh, India.
Era's Lucknow Medical University, Lucknow, Uttar Pradesh, India.
出版信息
J Med Biochem. 2020 Jan 23;39(2):165-170. doi: 10.2478/jomb-2019-0033.
BACKGROUND
Fibromyalgia syndrome (FMS) is characterized by altered pain perception with chronic, widespread musculoskeletal pain. The relationship between nitric oxide, oxidative stress and the severity of FMS has not been studied. This study evaluated NO levels in plasma, LPO products and antioxidants in Red Cell lysate in patients of FMS and correlated it with disease severity.
METHODS
105 FMS patients who fulfilled 1990 ACR Criteria and 105 age- and sex-matched healthy controls were recruited over two years from 2013 to 2015. Antioxidative enzyme activity was assessed by the estimation of catalase, glutathione peroxidase (GPx) and glutathione reductase (GR) and superoxide dismutase (SOD). Nitric oxide in plasma, MDA marker of lipid per - oxidation (LPO) in the lysate was donen for estimating oxidative stress. FIQR was used to assess the severity of fibromyalgia.
RESULTS
The catalase, superoxide dismutase, glutathione reductase and glutathione peroxidase levels were significantly low in patients than controls (p<0.001). Plasma NO levels and LPO were also significantly high (p<0.05). NO and LPO levels showed a significant positive correlation with FIQR (r: 0.57, 0.8 and p: <0.001) whereas a negative correlation was observed between antioxidants (Cat, GR and GPx, but not SOD) and FIQR.
CONCLUSIONS
Low antioxidants and raised LPO in RBC lysate in patients with FM together with high plasma NO correlated with the severity of FMS.
背景
纤维肌痛综合征（FMS）的特征是慢性广泛性肌肉骨骼疼痛导致痛觉改变。一氧化氮、氧化应激与FMS严重程度之间的关系尚未得到研究。本研究评估了FMS患者血浆中的一氧化氮水平、红细胞裂解物中的脂质过氧化产物（LPO）和抗氧化剂，并将其与疾病严重程度相关联。
方法
从2013年至2015年的两年间招募了105名符合1990年美国风湿病学会（ACR）标准的FMS患者以及105名年龄和性别匹配的健康对照者。通过测定过氧化氢酶、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）和超氧化物歧化酶（SOD）来评估抗氧化酶活性。检测血浆中的一氧化氮、裂解物中脂质过氧化的丙二醛（MDA）标志物以评估氧化应激。采用纤维肌痛影响问卷修订版（FIQR）评估纤维肌痛的严重程度。
结果
患者的过氧化氢酶、超氧化物歧化酶、谷胱甘肽还原酶和谷胱甘肽过氧化物酶水平显著低于对照组（p<0.001）。血浆一氧化氮水平和LPO也显著升高（p<0.05）。一氧化氮和LPO水平与FIQR呈显著正相关（r：0.57，0.8；p：<0.001），而抗氧化剂（过氧化氢酶、GR和GPx，但不包括SOD）与FIQR呈负相关。
结论
FM患者红细胞裂解物中抗氧化剂水平降低、LPO升高以及血浆一氧化氮水平升高与FMS的严重程度相关。
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