Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University, Seoul 02841, Republic of Korea.
School of Earth and Environmental Science and Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004930.
A Gram-stain-negative, aerobic, pale yellow-coloured, rod-shaped marine bacterium designated strain YJ-S2-02 was isolated from salt flat sediment sampled in Yongyu-do, Republic of Korea. Strain YJ-S2-02 grew at pH 6.0-9.0 (optimum, pH 7.0), 10-40 °C (optimum, 30 °C) and with optimum 1 % (w/v) NaCl. The 16S rRNA gene sequence analysis indicated that strain YJ-S2-02 was closely related to NBRC 102051 (97.8 %) followed by SM117 (97.5 %), H25 (97.3 %), US6-1 (96.8 %), STM-14 (96.6 %), EGI60015 (96.5 %), D39 (96.5 %) and MUSC 273 (95.9 %). The average nucleotide identity and estimated DNA-DNA hybridization values between YJ-S2-02 and related type strains were 77.0-77.9 % and 19.1-24.0 %. Strain YJ-S2-02 was characterized as having Q-10 as the predominant respiratory quinone and the principal fatty acids (>10 %) were summed feature 8 (C cω7, 20.7 %), C 6 (16.3 %) and C 6 (11.8 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingolipids and two unidentified lipids. The DNA G+C content of strain YJ-S2-02 was 65.6 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S2-02 should be classified as representing a novel species within the genus , for which name is proposed, with the type strain YJ-S2-02 (=KACC 21677 =KCTC 72891=JCM 33996).
一株革兰氏阴性、需氧、浅黄色、杆状海洋细菌，被命名为 YJ-S2-02 菌株，从韩国郁陵岛的盐滩沉积物中分离得到。YJ-S2-02 菌株在 pH6.0-9.0（最佳，pH7.0）、10-40°C（最佳，30°C）和最佳 1%（w/v）NaCl 下生长。16S rRNA 基因序列分析表明，YJ-S2-02 菌株与 NBRC 102051（97.8%）最为密切相关，其次是 SM117（97.5%）、H25（97.3%）、US6-1（96.8%）、STM-14（96.6%）、EGI60015（96.5%）、D39（96.5%）和 MUSC 273（95.9%）。YJ-S2-02 菌株与相关模式菌株之间的平均核苷酸同一性和估计的 DNA-DNA 杂交值分别为 77.0-77.9%和 19.1-24.0%。YJ-S2-02 菌株的特征是 Q-10 作为主要的呼吸醌，主要脂肪酸（>10%）为 summed feature 8（C cω7，20.7%）、C6（16.3%）和 C6（11.8%）。极性脂由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、鞘脂和两种未鉴定的脂组成。菌株 YJ-S2-02 的 DNA G+C 含量为 65.6 mol%。基于本研究中呈现的多相分类证据，YJ-S2-02 菌株应归类为代表属内的一个新种，建议使用 为其命名，其模式菌株为 YJ-S2-02 菌株（=KACC 21677 =KCTC 72891=JCM 33996）。
