Department of Neuroscience, Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06510, USA.
Department of Neuroscience, Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06510, USA.
Neuron. 2020 Oct 14;108(1):33-43. doi: 10.1016/j.neuron.2020.09.031.
Optical imaging has revolutionized our ability to monitor brain activity, spanning spatial scales from synapses to cells to circuits. Here, we summarize the rapid development and application of mesoscopic imaging, a widefield fluorescence-based approach that balances high spatiotemporal resolution with extraordinarily large fields of view. By leveraging the continued expansion of fluorescent reporters for neuronal activity and novel strategies for indicator expression, mesoscopic analysis enables measurement and correlation of network dynamics with behavioral state and task performance. Moreover, the combination of widefield imaging with cellular resolution methods such as two-photon microscopy and electrophysiology is bridging boundaries between cellular and network analyses. Overall, mesoscopic imaging provides a powerful option in the optical toolbox for investigation of brain function.
光学成像是监测大脑活动的一项重大突破，可以在从突触到细胞到回路的各种空间尺度上进行。在这里，我们总结了介观成像的快速发展和应用，这是一种基于宽场荧光的方法，在保持高时空分辨率的同时，还具有非常大的视野。通过利用不断扩展的用于神经元活动的荧光报告基因和新型指示剂表达策略，介观分析能够测量和关联网络动力学与行为状态和任务表现。此外，宽场成像与双光子显微镜和电生理学等细胞分辨率方法的结合，正在弥合细胞和网络分析之间的界限。总的来说，介观成像为大脑功能研究的光学工具包提供了一种强大的选择。
