Department of Electrical and Computer Engineering, University of California, Santa Barbara, CA, USA.
LifeCanvas Technologies, Cambridge, MA, USA.
Nat Commun. 2021 Nov 17;12(1):6639. doi: 10.1038/s41467-021-26736-4.
Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view of ~25 mm, encompassing distances up to 7 mm, with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.
在散射组织中对广泛分布于大脑区域的神经元的活动进行高速成像仍然具有挑战性。在这里，我们介绍了一种具有双独立增强扫描引擎的开源系统，用于大视场双光子成像（Diesel2p）。该系统结合了光学设计、自适应光学和时间复用技术，在~25 毫米的大视场范围内提供亚细胞分辨率，可涵盖长达 7 毫米的距离，具有独立的扫描引擎。我们展示了该系统在活体大脑神经元钙成像中的灵活性和各种应用案例。
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