Laboratório de Ecotoxicologia, Centro de Química e Meio Ambiente, Instituto de Pesquisas Energéticas e Nucleares (IPEN), Comissão Nacional de Energia Nuclear- IPEN/CNEN-SP, São Paulo, SP, Brazil.
Institute of Green Nanotechnology, Department of Radiology, University of Missouri Columbia, Columbia, MO, USA.
Int J Nanomedicine. 2020 Oct 2;15:7359-7376. doi: 10.2147/IJN.S250467. eCollection 2020.
We report, herein, in vitro, and in vivo toxicity evaluation of silver nanoparticles stabilized with gum arabic protein (AgNP-GP) in embryos and in Sprague Dawley rats.
The objective of this investigation was to evaluate in vitro and in vivo toxicity of silver nanoparticles stabilized with gum arabic protein (AgNP-GP), in multispecies due to the recognition that toxicity evaluations beyond a single species reflect the environmental realism. In the present study, AgNP-GP was synthesized through the reduction of silver salt using the tri-alanine-phosphine peptide (commonly referred to as "Katti Peptide") and stabilized using gum arabic protein.
In vitro cytotoxicity tests were performed according to ISO 10993-5 protocols to assess cytotoxicity index (IC) values. Acute ecotoxicity (EC) studies were performed using , according to the ABNT NBR 15088 protocols. In vivo toxicity also included evaluation of acute embryotoxicity using (zebrafish) embryos following the OECD No. 236 guidelines. We also used Sprague Dawley rats to assess the toxicity of AgNP-GP in doses from 2.5 to 10.0 mg kg body weight.
AgNP-GP nanoparticles were characterized through UV (405 nm), core size (20±5 nm through TEM), hydrodynamic size (70-80 nm), Zeta (ζ) potential (- 26 mV) using DLS and Powder X ray diffraction (PXRD) and EDS. PXRD showed pattern consistent with the Ag (1 1 1) peak. EC in was 4.40 (3.59-5.40) μg L. In the zebrafish species, LC was 177 μg L. Oral administration of AgNP-GP in Sprague Dawley rats for a period of 28 days revealed no adverse effects in doses of up to 10.0 mg kg b.w. in both male and female animals.
The non-toxicity of AgNP-GP in rats offers a myriad of applications of AgNP-GP in health and hygiene for use as antibiotics, antimicrobial and antifungal agents.
我们报告了在此处使用阿拉伯树胶蛋白稳定的银纳米粒子（AgNP-GP）在胚胎和 Sprague Dawley 大鼠中的体外和体内毒性评估。
本研究的目的是评估通过单一物种之外的毒性评估来反映环境现实的情况下，使用阿拉伯树胶蛋白稳定的银纳米粒子（AgNP-GP）的多物种的体外和体内毒性。在本研究中，通过使用三丙氨酸-膦肽（通常称为“Katti 肽”）还原银盐并使用阿拉伯树胶蛋白稳定来合成 AgNP-GP。
根据 ISO 10993-5 协议进行体外细胞毒性测试，以评估细胞毒性指数（IC）值。根据 ABNT NBR 15088 协议进行急性生态毒性（EC）研究。根据 OECD 第 236 号准则进行体内毒性评估，包括使用斑马鱼胚胎进行急性胚胎毒性评估。我们还使用 Sprague Dawley 大鼠评估 AgNP-GP 在 2.5 至 10.0 mg kg 体重剂量下的毒性。
通过 UV（405nm）、TEM 中的核心尺寸（20±5nm）、动态尺寸（70-80nm）、Zeta（ζ）电势（-26mV）通过 DLS 和粉末 X 射线衍射（PXRD）和 EDS 对 AgNP-GP 纳米粒子进行了表征。PXRD 显示出与 Ag（111）峰一致的图案。在 中的 EC 为 4.40（3.59-5.40）μg L。在斑马鱼物种中，LC 为 177μg L。在 Sprague Dawley 大鼠中口服给予 AgNP-GP 长达 28 天，在雄性和雌性动物中，高达 10.0mg kg b.w.的剂量均未显示出不良反应。
AgNP-GP 在大鼠中的非毒性为 AgNP-GP 在健康和卫生领域的广泛应用提供了可能性，可作为抗生素、抗菌和抗真菌剂。
