Doheny Image Reading Center, Doheny Eye Institute, Los Angeles, CA, USA.
Department of Ophthalmology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
Transl Vis Sci Technol. 2020 Sep 28;9(10):29. doi: 10.1167/tvst.9.10.29. eCollection 2020 Sep.
The purpose of this study was to evaluate and compare the repeatability of optical coherence tomography angiography (OCTA) derived retinal vascular quantitative metrics using high resolution (HR) versus high speed (HS) acquisition modes.
Macular 4.4 × 2.9-mm OCTA images from normal, healthy volunteers were captured using both HR (768 A-scans × 256 B-scans) and HS (384 A-scans × 256 B-scans) acquisition protocols. Vessel density and vessel length density of the superficial capillary plexus and deep capillary plexus, as well as choriocapillaris flow deficit were computed. In a subset of eyes, the OCTA scans were repeated twice 2 days later and the same metrics were recomputed to assess repeatability, using intraclass correlation coefficients and coefficient of variation (CV).
Comparison of measurements between HR and HS acquisitions in 41 healthy eyes showed statistically significant differences for all quantitative metrics ( < 0.01). However, no significant differences were observed among the three repeated scans (one on day 1 and two on day 3) obtained with each of the acquisition modes. The CVs ranged from 0.01 to 0.03 with the HR mode and 0.01 to 0.04 with the HS mode. The intraclass correlation coefficients (ICCs) among measurements were similar and high for both HR and HS modes, ranging from 0.844 to 0.949.
HR and HS OCTA acquisition modes both yielded repeatable quantitative metrics for the retinal circulation and the choriocapillaris. However, despite a similar field, the HR and HS derived measurements differed significantly.
To establish OCTA imaging in clinical and research practice, standardized and repeatability image analysis are necessary.
本研究旨在评估和比较使用高分辨率（HR）与高速（HS）采集模式获得的光学相干断层扫描血管造影（OCTA）视网膜血管定量指标的可重复性。
使用 HR（768 个 A 扫描×256 个 B 扫描）和 HS（384 个 A 扫描×256 个 B 扫描）采集方案对正常、健康志愿者的黄斑 4.4×2.9-mm OCTA 图像进行采集。计算浅层毛细血管丛和深层毛细血管丛的血管密度和血管长度密度以及脉络膜毛细血管血流不足。在部分眼中，两次在 2 天后重复 OCTA 扫描，并使用组内相关系数和变异系数（CV）重新计算相同的指标以评估重复性。
在 41 只健康眼中，HR 和 HS 采集之间的测量值比较显示所有定量指标均存在统计学差异（<0.01）。然而，在每种采集模式下获得的三次重复扫描（第一天一次，第三天两次）之间未观察到显著差异。CV 范围为 HR 模式 0.01 至 0.03，HS 模式 0.01 至 0.04。HR 和 HS 模式的测量值之间的组内相关系数（ICC）相似且较高，范围分别为 0.844 至 0.949。
HR 和 HS OCTA 采集模式均可获得视网膜循环和脉络膜毛细血管的可重复定量指标。然而，尽管视野相似，但 HR 和 HS 衍生的测量值差异显著。
杨梦涵
刘欢
