基于 PCR 的检测方法以 Hock-a-loogie 唾液作为 SARS-CoV-2 的诊断标本：一项诊断有效性研究。
Hock-a-loogie saliva as a diagnostic specimen for SARS-CoV-2 by a PCR-based assay: A diagnostic validity study.
机构信息
Department of Laboratory Medicine, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, PR China; Institute of Laboratory Medicine, Zhejiang University, Hangzhou, PR China.
Department of Laboratory Medicine, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, PR China; Institute of Laboratory Medicine, Zhejiang University, Hangzhou, PR China.
出版信息
Clin Chim Acta. 2020 Dec;511:177-180. doi: 10.1016/j.cca.2020.10.004. Epub 2020 Oct 15.
To clarify the effect of different respiratory sample types on SARS-CoV-2 detection, we collected throat swabs, nasal swabs and hock-a-loogie saliva or sputum, and compared their detection rates and viral loads. The detection rates of sputum (95.65%, 22/23) and hock-a-loogie saliva (88.09%, 37/42) were significantly higher than those in throat swabs (41.54%, 27/65) and nasal swabs (72.31%, 47/65) (P < 0.001). The Ct Values of sputum, hock-a-loogie saliva and nasal swabs were significantly higher than that in throat swabs, whereas no significant difference was observed between sputum and saliva samples. Hock-a-loogie saliva are reliable sample types that can be used to detect SARS-CoV-2, and worthy of clinical promotion.
为了阐明不同呼吸道样本类型对 SARS-CoV-2 检测的影响，我们收集了咽拭子、鼻拭子和深咳痰液或痰液，并比较了它们的检出率和病毒载量。痰液（95.65%，22/23）和深咳痰液（88.09%，37/42）的检出率明显高于咽拭子（41.54%，27/65）和鼻拭子（72.31%，47/65）（P<0.001）。痰液、深咳痰液和鼻拭子的 Ct 值明显高于咽拭子，而痰液和唾液样本之间没有显著差异。深咳痰液是可靠的可用于检测 SARS-CoV-2 的样本类型，值得临床推广。
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