Department of Medical Microbiology and Medical Immunology, Meander Medical Center, Amersfoort, the Netherlands.
Department of Medical Microbiology and Medical Immunology, Meander Medical Center, Amersfoort, the Netherlands.
J Clin Virol. 2020 Dec;133:104655. doi: 10.1016/j.jcv.2020.104655. Epub 2020 Oct 5.
Correct and reliable identification of SARS-CoV-2 in COVID-19 suspected patients is essential for diagnosis. Respiratory samples should always be tested with real-time PCR for SARS-CoV-2. In addition, blood samples have been tested, but without consistent results and therefore the added value of this sample type is unknown. The aim of this study was to determine the prevalence of SARS-CoV-2 by real-time PCR in blood samples obtained from PCR-proven COVID-19 patients and in addition to elaborate on the potential use of blood for diagnostics. In this single center study, blood samples drawn from patients at the emergency department with proven COVID-19 infection based on a positive SARS-CoV-2 PCR in respiratory samples were tested for the presence of SARS-CoV-2. Samples from 118 patients were selected, of which 102 could be included in the study (median age was 65 (IQR 10), 65.7 % men). In six (5.9 %) of the tested samples, SARS-CoV-2 was identified by real-time PCR. In conclusion, SARS-CoV-2 can be detected by real-time PCR in plasma samples from patients with proven COVID-19, but only in a minority of the patients. Plasma should therefore not be used as primary sample in an acute phase setting to identify SARS-CoV-2 infection. These findings are important to complete the knowledge on possible sample types to test to diagnose COVID-19.
在 COVID-19 疑似患者中正确可靠地识别 SARS-CoV-2 对于诊断至关重要。应始终使用实时 PCR 对呼吸道样本进行 SARS-CoV-2 检测。此外，已经对血液样本进行了检测，但结果不一致，因此这种样本类型的附加值尚不清楚。本研究旨在通过对经实时 PCR 证实的 COVID-19 患者的血液样本进行检测，确定 SARS-CoV-2 的流行率，并进一步阐述血液用于诊断的潜力。在这项单中心研究中，从急诊科的 COVID-19 感染患者的血液样本中提取样本，这些患者的呼吸道样本中的 SARS-CoV-2 PCR 检测结果为阳性，用于检测 SARS-CoV-2 的存在。选择了 118 名患者的样本，其中 102 名可以纳入研究（中位数年龄为 65（IQR 10），65.7%为男性）。在 6 份（5.9%）检测样本中，通过实时 PCR 鉴定出 SARS-CoV-2。总之，从经实时 PCR 证实的 COVID-19 患者的血浆样本中可以检测到 SARS-CoV-2，但只有少数患者如此。因此，在急性阶段不应该将血浆用作识别 SARS-CoV-2 感染的主要样本。这些发现对于完善可能用于检测 COVID-19 的样本类型的知识非常重要。
