Department of Materials Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan.
Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan.
J Biomater Sci Polym Ed. 2021 Mar;32(4):419-437. doi: 10.1080/09205063.2020.1839340. Epub 2020 Nov 6.
Water-soluble photoreactive polymers with both phosphorylcholine and benzophenone groups were synthesized for the reaction between the polymers and the substrate in aqueous medium. To control the polymer architecture, the living radical polymerization method was applied to the copolymerization of 2-methacryloyloxyethyl phosphorylcholine and benzophenone methacrylates. These polymers possess various architectures, such as linear polymers, polymers with hydrophobic terminals, and 4-armed star-like polymers, that could promote their adsorption on the substrate surfaces. Additionally, two types of benzophenone groups were examined. Due to the bulky phosphorylcholine group, tetra(ethylene oxide) group as a spacer between polymer main chain and benzophenone group was considered. These polymers could adsorb on the surface in an aqueous medium, followed by reaction on the surface photoirradiation depending on the chemical structure of the benzophenone group. The thickness of the polymer layer depended on the polymer architecture, i.e. a polymer with a hydrophobic terminal could form a thick layer. After modification, the contact angle by air in the aqueous medium decreased, compared to that on the base substrate. This was due to the hydrophilic nature based on the phosphorylcholine groups at the surface. The amount of proteins adsorbed on the surface also decreased because of the surface modification. These findings indicated that these water-soluble photoreactive polymers could be applied for the safer and effective surface modification of substrates conventional photoirradiation without using an organic solvent.
合成了同时具有膦酸胆碱和苯甲酮基团的水溶性光反应性聚合物，以在水介质中实现聚合物与基底之间的反应。为了控制聚合物的结构，应用活性自由基聚合方法对 2-甲基丙烯酰氧乙基膦酸胆碱和苯甲酮甲基丙烯酸酯的共聚合进行了研究。这些聚合物具有各种结构，如线性聚合物、具有疏水性末端的聚合物和 4 臂星型聚合物，这可以促进它们在基底表面的吸附。此外，还研究了两种类型的苯甲酮基团。由于膦酸胆碱基团较大，考虑在聚合物主链和苯甲酮基团之间使用四（环氧乙烷）基团作为间隔基。这些聚合物可以在水介质中吸附在表面上，然后根据苯甲酮基团的化学结构进行表面光辐照反应。聚合物层的厚度取决于聚合物的结构，即具有疏水性末端的聚合物可以形成较厚的层。经过修饰后，与基底相比，在水介质中空气的接触角减小。这是由于表面上的亲水性质基于膦酸胆碱基团。由于表面修饰，吸附在表面上的蛋白质数量也减少了。这些发现表明，这些水溶性光反应性聚合物可以应用于更安全、有效的基底表面改性，而无需使用有机溶剂进行常规光辐射。
