Walchak Robert C, Buckwalter Seanne P, Zinsmaster Nicole M, Henn Katrina M, Johnson Katelyn M, Koelsch Jolene M, Herring Senait A, Steinmetz Lory K, Reed Katelyn A, Barth Jean E, Rasmusson Jenna M, Fischer Jill L, Snippes Vagnone Paula, Sampathkumar Priya, Wengenack Nancy L
Division of Clinical Microbiology, Mayo Clinic, Rochester, MN 55902, USA.
Research and Innovation Office, Mayo Clinic, Rochester, MN 55902, USA.
J Fungi (Basel). 2020 Oct 15;6(4):224. doi: 10.3390/jof6040224.
is an emerging fungal pathogen with cases reported in countries around the world and in 19 states within the United States as of August 2020. The CDC has recommended that hospitals perform active surveillance upon admission for patients with the appropriate risk factors. Currently, active surveillance requires that local hospitals send surveillance swabs to a public health laboratory for analysis. In this work, a real-time PCR assay was developed for the specific detection of from surveillance swabs, blood, and urine to enable rapid detection of this pathogen. The assay uses commercially available primers and reporter probes and it was verified on the LightCycler 480 PCR platform. Contrived specimens and prospectively collected composite groin/axilla surveillance swabs were used to validate the assay. The performance of the PCR assay on surveillance swabs was also compared to a second PCR assay targeting that was performed at the Minnesota Department of Health-Public Health Laboratory (MDH-PHL). Our PCR assay is able to detect and differentiate from closely related species such as , , and on the basis of melting curve temperature differences.
是一种新兴的真菌病原体，截至2020年8月，全球多个国家以及美国19个州都报告了相关病例。美国疾病控制与预防中心建议医院对有适当风险因素的入院患者进行主动监测。目前，主动监测要求当地医院将监测拭子送至公共卫生实验室进行分析。在这项工作中，开发了一种实时PCR检测方法，用于从监测拭子、血液和尿液中特异性检测，以便能够快速检测出这种病原体。该检测方法使用市售引物和报告探针，并在LightCycler 480 PCR平台上进行了验证。使用人工制备的样本和前瞻性收集的腹股沟/腋窝复合监测拭子对该检测方法进行验证。还将该PCR检测方法在监测拭子上的性能与明尼苏达州卫生部公共卫生实验室（MDH-PHL）进行的针对的第二种PCR检测方法进行了比较。我们的PCR检测方法能够根据熔解曲线温度差异，从、和等密切相关的物种中检测和区分出。
