Sattler Janko, Noster Janina, Brunke Anne, Plum Georg, Wiegel Pia, Kurzai Oliver, Meis Jacques F, Hamprecht Axel
Institute for Medical Microbiology, Immunology and Hygiene, University Hospital of Cologne, University of Cologne, 50935 Cologne, Germany.
German Centre for Infection Research, Partner Site Bonn-Cologne, 50937 Cologne, Germany.
J Fungi (Basel). 2021 Feb 22;7(2):154. doi: 10.3390/jof7020154.
is an emerging pathogen with resistance to many commonly used antifungal agents. Infections with require rapid and reliable detection methods to initiate successful medical treatment and contain hospital outbreaks. Conventional identification methods are prone to errors and can lead to misidentifications. PCR-based assays, in turn, can provide reliable results with low turnaround times. However, only limited data are available on the performance of commercially available assays for detection. In the present study, the two commercially available PCR assays ID (OLM, Newcastle Upon Tyne, UK) and Fungiplex RUO Real-Time PCR (Bruker, Bremen, Germany) were challenged with 29 isolates from all five clades and eight other species as controls. ID reliably detected with a limit of detection (LoD) of 1 genome copies/reaction. However, false positive results were obtained with high DNA amounts of the closely related species and . The Fungiplex RUO Real-Time PCR kit detected with an LoD of 9 copies/reaction. No false positive results were obtained with this assay. In addition, could also be detected in human blood samples spiked with pure fungal cultures by both kits. In summary, both kits could detect -DNA at low DNA concentrations but differed slightly in their limits of detection and specificity.
是一种对许多常用抗真菌药物具有抗性的新兴病原体。感染需要快速可靠的检测方法来启动成功的医学治疗并控制医院内的疫情爆发。传统的鉴定方法容易出错并可能导致错误鉴定。基于PCR的检测方法则可以在较短的周转时间内提供可靠的结果。然而，关于用于检测的市售检测方法的性能，仅有有限的数据。在本研究中，用来自所有五个进化枝的29株分离株以及作为对照的其他八个物种对两种市售的PCR检测方法ID（OLM，英国泰恩河畔纽卡斯尔）和Fungiplex RUO实时PCR（布鲁克，德国不来梅）进行了测试。ID能够可靠地检测，检测限（LoD）为1个基因组拷贝/反应。然而，对于密切相关的物种和，在DNA量较高时获得了假阳性结果。Fungiplex RUO实时PCR试剂盒检测的LoD为9个拷贝/反应。该检测方法未获得假阳性结果。此外，两种试剂盒均可在接种了纯真菌培养物的人血样本中检测到。总之，两种试剂盒均可在低DNA浓度下检测到-DNA，但它们的检测限和特异性略有不同。
