Chaturvedi Vivek K, Rai Sachchida Nand, Tabassum Nazish, Yadav Navneet, Singh Veer, Bohara Raghvendra A, Singh Mohan P
Centre of Biotechnology, University of Allahabad, Prayagraj, 211002, India.
Department of Physics, University of Allahabad, Prayagraj, 211002, India.
Biochem Biophys Rep. 2020 Oct 5;24:100812. doi: 10.1016/j.bbrep.2020.100812. eCollection 2020 Dec.
In the current scenario of the fight against cancer Integration of potential elements seems to be the best alternative since it overcomes the weaknesses of individuals and the combination of elements makes them formidable in the fight against the cancer war. Inspired by this objective and trusting our knowledge of paddy straw grown oyster mushroom, (Pf) mediated synthesis; a first-of-kind approach has been developed for the rapid synthesis of Au-Pt-Ag trimetallic nanoparticles (TMNPs). The developed method was successful, which was confirmed by Ultraviolet-Visible, X-ray diffraction, Transmission Electron Microscopy, Energy Dispersive Spectroscopy. Specifically, prepared TMNPs have been studied for their stability and size as a primary prerequisite for nanomedicine. Finally, the stable nanomedicine developed has been assessed for its performance against the highly metastatic breast cancer cell line (mda-mb-231). The performance was assessed using MTT assay and morphological readings, which were integrated with the cell viability data. We also determined the IC50 value, which was far superior to individual components and motivated us to postulate the possible breast cancer cell killing mechanism of TMNPs. The present study unlocks the new paths for the mushroom-mediated environmentally friendly, economic synthesis of trimetallic nanoparticles, which can be effectively used in cancer nanomedicine.
在当前抗癌斗争的形势下，整合潜在要素似乎是最佳选择，因为它克服了单个要素的弱点，要素的组合使其在抗癌之战中变得强大。受这一目标的启发，并基于我们对稻草栽培平菇（Pf）介导合成的了解，开发了一种首创的方法来快速合成金-铂-银三金属纳米颗粒（TMNPs）。所开发的方法是成功的，这通过紫外可见光谱、X射线衍射、透射电子显微镜、能量色散光谱得到了证实。具体而言，已对制备的TMNPs的稳定性和尺寸进行了研究，这是纳米医学的主要先决条件。最后，对所开发的稳定纳米药物针对高转移性乳腺癌细胞系（mda-mb-231）的性能进行了评估。使用MTT法和形态学读数对性能进行了评估，并将其与细胞活力数据相结合。我们还确定了IC50值，该值远优于单个成分，并促使我们推测TMNPs可能的乳腺癌细胞杀伤机制。本研究为蘑菇介导的环境友好、经济的三金属纳米颗粒合成开辟了新途径，其可有效地用于癌症纳米医学。
