骆驼囊型棘球蚴病的分子研究（）及伊朗的报告
Molecular Studies on Cystic Echinococcosis of Camel () and Report of in Iran.
作者信息
Ebrahimipour Mohammad, Sadjjadi Seyed Mahmoud, Yousofi Darani Hossein, Najjari Mohsen
机构信息
Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Iran J Parasitol. 2017 Jul-Sep;12(3):323-331.
BACKGROUND
Cystic echinococcosis (CE) is one of the most important zoonotic diseases; caused by different genotypes of spp. Camels have an important role in transmission cycle of especially, in desert areas. This study aimed to investigate molecular characterization of hydatid cysts isolates from one-humped camel ( and to show its molecular and phylogenic status in this important CE host in the central part of Iran.
METHODS
Twenty hydatid cyst samples (14 fertile and 6 calcified) were collected from 56 slaughtered camels in Central part of Iran. Extraction of DNA from 14 fertile samples was achieved followed by molecular studies on two mitochondrial genes ( and ).
RESULTS
Blast and phylogenetic analysis on sequenced genes showed the presence of G1 (28.6%), G3 (28.6%) and G6 (35.7%) genotypes in the samples. However, one sample was detected as (G5) with 99% homology with G5 isolated from camel in Egypt (AB921055) and Sudan (JX912709).
CONCLUSION
Presence of , originally the cattle genotype, is reported for the first time in Iran. Due to the potential of infecting human by ; more attention should be paid to this zoonotic genotype in this region.
背景
囊型包虫病（CE）是最重要的人畜共患病之一，由不同基因型的棘球绦虫属物种引起。骆驼在棘球绦虫的传播循环中起着重要作用，尤其是在沙漠地区。本研究旨在调查单峰骆驼体内包虫囊肿分离株的分子特征，并展示其在伊朗中部这一重要的囊型包虫病宿主中的分子和系统发育地位。
方法
从伊朗中部56头屠宰骆驼中采集了20个包虫囊肿样本（14个有活力的和6个钙化的）。从14个有活力的样本中提取DNA，随后对两个线粒体基因（和）进行分子研究。
结果
对测序基因的Blast和系统发育分析表明，样本中存在G1（28.6%）、G3（28.6%）和G6（35.7%）基因型。然而，有一个样本被检测为G5，与从埃及骆驼（AB921055）和苏丹骆驼（JX912709）分离出的G5具有99%的同源性。
结论
伊朗首次报道了原本为牛基因型的G5的存在。由于G5有感染人类的可能性，该地区应更加关注这种人畜共患病基因型。
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