Antibacterial property of , , and against oral microbes.

CONTEXT

Plant extracts are used in folklore medicine from time immemorial to treat different oral diseases. Chemical constituents extracted from these natural resources are gifted with huge opportunities.

AIM

The aim of this study is to assess the antibacterial property of (Neem), (Tulsi), and (Pochotia) against oral microorganisms.

MATERIALS AND METHODS

Plant extract was prepared with hot continuous extraction method by the Soxhlet Apparatus. Microorganisms isolated from the oral cavity and identified by Vitek-2. Bacterial inoculums poured and spread into Mueller Hinton plates. Plant extract was poured into prepared wells taking ciprofloxacillin as the positive control and dimethyl sulfoxide as the negative control. The experiment was performed in duplicates with two different concentrations of the extract and mean value of inhibition zone was calculated.

STATISTICAL ANALYSIS

Paired -test, analysis of variance, and regression analysis.

RESULTS

Isolated microorganisms were , and . Higher zone of inhibition was observed against by followed by . Among the aqueous and acetone group, in the aqueous group, the regression models of and have been found to be statistically significant (= < 0.05), whereas, in the acetone group, the regression model of has been found to be statistically significant ( = < 0.05).

CONCLUSION

All the three plants showed antibacterial potency against the isolated organisms. Acetone group showed better efficacy than the aqueous extract group.