Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
Molecules. 2020 Oct 21;25(20):4862. doi: 10.3390/molecules25204862.
Using antibiotics as feed additives have been successively banned worldwide from 1986; therefore, it is an urgent task to finding safe and effective alternatives. As natural products of plant origin, essential oils (EOs) are an outstanding option due to their reported bioactivity. In this research, ten EOs of species were extracted by steam distillation and its chemical constituents were identified by gas chromatography-mass spectrometry (GC-MS). A total of 123 chemical compounds, including alkenes, phenols, aldehydes and ketones, were identified. The results of antioxidant activity carried out through DPPH free radical scavenging (DPPH) and ferric reducing antioxidant power (FRAP), showing that EOs of Linn. (ObEO), Ronn. (TmEO), Linn. (OvEO) and Maxim. (McEO) have strong antioxidant activities. Their 50%-inhibitory concentration (IC) value was <1.00, 1.42, 1.47 and 1.92 μg/mL, respectively; and their FRAP value was 1536.67 ± 24.22, 271.84 ± 4.93, 633.71 ± 13.14 and 480.66 ± 29.90, respectively. The results of filter paper diffusion showing that McEO, OvEO and TmEO inhibition zone diameter (IZD) are all over 30 mm. The results of two-fold dilution method showed that McEO, OvEO and TmEO have strong antibacterial activities against and their minimal inhibitory concentrations (MIC) value was 1 μL/mL, 2 μL/mL, and 2 μL/mL, respectively. In conclusion, the results in this work demonstrate the possibility for development and application of EOs as potential feed additives.
自 1986 年以来，抗生素作为饲料添加剂已在全球范围内相继被禁止使用；因此，寻找安全有效的替代品是当务之急。由于植物源性天然产物精油（EOs）具有报道的生物活性，因此作为潜在的饲料添加剂具有广阔的开发和应用前景。在这项研究中，采用水蒸气蒸馏法提取了 10 种 物种的精油，并通过气相色谱-质谱联用仪（GC-MS）鉴定了其化学成分。共鉴定出 123 种化合物，包括烯烃、酚类、醛类和酮类。通过 DPPH 自由基清除（DPPH）和铁还原抗氧化能力（FRAP）测定抗氧化活性的结果表明， Linn.（ObEO）、 Ronn.（TmEO）、 Linn.（OvEO）和 Maxim.（McEO）精油具有较强的抗氧化活性。它们的 50%-抑制浓度（IC）值分别为<1.00、1.42、1.47 和 1.92 μg/mL；FRAP 值分别为 1536.67 ± 24.22、271.84 ± 4.93、633.71 ± 13.14 和 480.66 ± 29.90。滤纸扩散结果表明，McEO、OvEO 和 TmEO 的抑菌圈直径（IZD）均超过 30mm。二倍稀释法结果表明，McEO、OvEO 和 TmEO 对 和 均具有较强的抗菌活性，其最小抑菌浓度（MIC）值分别为 1 μL/mL、2 μL/mL 和 2 μL/mL。综上所述，本研究结果表明 EOs 作为潜在的饲料添加剂具有开发和应用的可能性。
