Kim B S, Kim S-H, Kim K, An Y-H, So K-H, Kim B-G, Hwang N S
Interdisciplinary Program in Bioengineering, Seoul National University, Republic of Korea.
School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Republic of Korea.
Mater Today Bio. 2020 Sep 21;8:100079. doi: 10.1016/j.mtbio.2020.100079. eCollection 2020 Sep.
Polyphenols can trigger immunity that activates intracellular anti-inflammatory signaling and prevents external infections. In this study, we report the fabrication of chitosan-based hydrogels with epigallocatechin gallate (EGCG) using enzyme-mediated one-pot synthesis. The tyrosinase-mediated oxidative reaction of the phenolic rings of EGCG with the primary amines on chitosan results in stable EGCG-chitosan hydrogels. The EGCG concentrations contributed to the cross-linking density and physical properties of EGCG-chitosan hydrogels. Furthermore, EGCG-chitosan hydrogels maintained intrinsic properties such as antibacterial and antioxidant effects. When endotoxin-activated RAW 264.7 macrophage cells were cultured with EGCG-chitosan hydrogels, the hydrogels reduced the inflammatory response of the RAW 264.7 cells. Furthermore, subcutaneous implantation of EGCG-chitosan hydrogels reduced endogenous macrophage and monocyte activation. When the EGCG-chitosan hydrogels were applied to a full-skin defect wound, they facilitated skin regeneration. Our study demonstrates that the one-pot synthesized EGCG-chitosan hydrogels can be applied in broad tissue regeneration applications that require immune modulation.
多酚可触发免疫反应，激活细胞内抗炎信号并预防外部感染。在本研究中，我们报道了使用酶介导的一锅法合成制备含表没食子儿茶素没食子酸酯（EGCG）的壳聚糖基水凝胶。EGCG酚环与壳聚糖上伯胺的酪氨酸酶介导的氧化反应产生了稳定的EGCG-壳聚糖水凝胶。EGCG浓度影响了EGCG-壳聚糖水凝胶的交联密度和物理性质。此外，EGCG-壳聚糖水凝胶保持了抗菌和抗氧化等固有特性。当用EGCG-壳聚糖水凝胶培养内毒素激活的RAW 264.7巨噬细胞时，水凝胶降低了RAW 264.7细胞的炎症反应。此外，皮下植入EGCG-壳聚糖水凝胶减少了内源性巨噬细胞和单核细胞的激活。当将EGCG-壳聚糖水凝胶应用于全层皮肤缺损伤口时，它们促进了皮肤再生。我们的研究表明，一锅法合成的EGCG-壳聚糖水凝胶可应用于需要免疫调节的广泛组织再生应用中。
