Genetics and Plant Breeding Section, Department of Soil, Plant and Food Sciences (DiSSPA), University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy.
Institute of Biosciences and Bioresources, National Research Council, Via Amendola 165/A, 70126 Bari, Italy.
Int J Mol Sci. 2020 Oct 24;21(21):7910. doi: 10.3390/ijms21217910.
Wheat is the most widely grown crop and represents the staple food for one third of the world's population. Wheat is attacked by a large variety of pathogens and the use of resistant cultivars is an effective and environmentally safe strategy for controlling diseases and eliminating the use of fungicides. In this study, a collection of wild and cultivated tetraploid wheats ( were evaluated for seedling resistance (SR) and adult plant resistance (APR) to powdery mildew () and genotyped with a 90K single nucleotide polymorphism (SNP) array to identify new sources of resistance genes. The genome-wide association mapping detected 18 quantitative trait loci (QTL) for APR and 8 QTL for SR, four of which were identical or at least closely linked to four QTL for APR. Thirteen candidate genes, containing nucleotide binding sites and leucine-rich repeats, were localized in the confidence intervals of the QTL-tagging SNPs. The marker IWB6155, associated to was located within the gene coding for a disease resistance protein. While most of the identified QTL were described previously, five QTL for APR and three QTL for SR () were mapped on chromosome regions where no resistance gene was reported before. The novel QTL/genes can contribute to enriching the resistance sources available to breeders.
小麦是种植最广泛的作物，代表了全球三分之一人口的主食。小麦受到多种病原体的攻击，利用抗性品种是控制病害和消除杀菌剂使用的有效和环境安全策略。在这项研究中，对野生和栽培四倍体小麦（）进行了评价，以评估其对白粉病的幼苗抗性（SR）和成株抗性（APR），并利用 90K 单核苷酸多态性（SNP）阵列进行基因分型，以鉴定新的抗性基因来源。全基因组关联图谱检测到 APR 的 18 个数量性状位点（QTL）和 SR 的 8 个 QTL，其中 4 个与 APR 的 4 个 QTL 相同或至少紧密连锁。在 QTL 标记 SNP 的置信区间内定位了包含核苷酸结合位点和富含亮氨酸重复序列的 13 个候选基因。与 相关的标记 IWB6155 位于编码抗病蛋白的基因内。虽然大多数鉴定的 QTL 以前都有描述，但 APR 的 5 个 QTL 和 SR 的 3 个 QTL 被映射到以前没有报道过抗性基因的染色体区域。新的 QTL/基因可以丰富育种者可用的抗性来源。
