Li Yuyang, Saladino Giovanni M, Shaker Kian, Svenda Martin, Vogt Carmen, Brodin Bertha, Hertz Hans M, Toprak Muhammet S
Biomedical and X-ray Physics, Department of Applied Physics, KTH Royal Institute of Technology, SE 10691 Stockholm, Sweden.
Nanomaterials (Basel). 2020 Oct 27;10(11):2129. doi: 10.3390/nano10112129.
Morphologically controllable synthesis of Rh nanoparticles (NPs) was achieved by the use of additives during polyol synthesis. The effect of salts and surfactant additives including PVP, sodium acetate, sodium citrate, CTAB, CTAC, and potassium bromide on Rh NPs morphology was investigated. When PVP was used as the only additive, trigonal NPs were obtained. Additives containing Br ions (CTAB and KBr) resulted in NPs with a cubic morphology, while those with carboxyl groups (sodium citrate and acetate) formed spheroid NPs. The use of Cl ions (CTAC) resulted in a mixture of polygon morphologies. Cytotoxicity of these NPs was evaluated on macrophages and ovarian cancer cell lines. Membrane integrity and cellular activity are both influenced to a similar extent, for both the cell lines, with respect to the morphology of Rh NPs. The cells exposed to trigonal Rh NPs showed the highest viability, among the NP series. Particles with a mixed polygon morphology had the highest cytotoxic impact, followed by cubic and spherical NPs. The Rh NPs were further demonstrated as contrast agents for X-ray fluorescence computed tomography (XFCT) in a small-animal imaging setting. This work provides a detailed route for the synthesis, morphology control, and characterization of Rh NPs as viable contrast agents for XFCT bio-imaging.
在多元醇合成过程中，通过使用添加剂实现了对铑纳米颗粒（NPs）形态的可控合成。研究了盐类和表面活性剂添加剂（包括聚乙烯吡咯烷酮（PVP）、醋酸钠、柠檬酸钠、十六烷基三甲基溴化铵（CTAB）、十六烷基三甲基氯化铵（CTAC）和溴化钾）对铑纳米颗粒形态的影响。当仅使用PVP作为添加剂时，可获得三角形纳米颗粒。含溴离子的添加剂（CTAB和KBr）可得到立方形态的纳米颗粒，而含羧基的添加剂（柠檬酸钠和醋酸钠）则形成球形纳米颗粒。使用氯离子（CTAC）会导致多种多边形形态的混合物。对这些纳米颗粒在巨噬细胞和卵巢癌细胞系上的细胞毒性进行了评估。对于这两种细胞系，膜完整性和细胞活性受到的影响程度相似，这与铑纳米颗粒的形态有关。在纳米颗粒系列中，暴露于三角形铑纳米颗粒的细胞显示出最高的活力。具有混合多边形形态的颗粒具有最高的细胞毒性影响，其次是立方形态和球形纳米颗粒。在小动物成像环境中，铑纳米颗粒进一步被证明可作为X射线荧光计算机断层扫描（XFCT）的造影剂。这项工作为铑纳米颗粒作为XFCT生物成像的可行造影剂的合成、形态控制和表征提供了详细途径。
