Kaufmann C S, Merz W G
Department of Laboratory Medicine (Pathology), Johns Hopkins Medical Institutions, Baltimore, Maryland 21205.
J Clin Microbiol. 1989 Oct;27(10):2165-8. doi: 10.1128/jcm.27.10.2165-2168.1989.
Chromosome-sized DNA molecules of clinical isolates of Torulopsis glabrata were resolved by a pulsed-field electrophoretic method, contour-clamped homogeneous electric fields. With the conditions established in this study, 8 to 12 bands (ranging from 445 to 3,000 kilobases) were observed. There were differences in the intensities and migrations of bands, consistent with T. glabrata being either haploid or diploid. A total of 22 distinctive electrophoretic patterns were noted among single isolates of T. glabrata recovered from 33 patients. When strains were delineated by an electrophoretic pattern, individuals usually harbored only one strain.
光滑球拟酵母临床分离株的染色体大小的DNA分子通过一种脉冲场电泳方法——轮廓夹恒定均匀电场得以解析。在本研究建立的条件下，观察到8至12条带（范围从445至3000千碱基）。各条带的强度和迁移存在差异，这与光滑球拟酵母为单倍体或二倍体一致。从33名患者中分离得到的光滑球拟酵母单株中共记录到22种独特的电泳图谱。当根据电泳图谱划分菌株时，个体通常仅携带一种菌株。
