Cao Kun-Kun, Zhang Sha-Sha, Hu Xue-Yu, Huang Yang
School of Environmental Studies, China University of Geosciences, Wuhan 430078, China.
Huan Jing Ke Xue. 2020 Nov 8;41(11):5185-5192. doi: 10.13227/j.hjkx.202004160.
To explore the effects of biochar on the temperature sensitivity of soil respiration and microbial community structure, the soil was subjected to an indoor culture test under two major treatments which were control (CK) and the addition of 3% (mass ratio) biochar (BC). Each major treatment contained four temperature conditions. We analyzed the changes in soil CO emission, the content of different soil organic carbon, and the characteristics of the soil bacterial community. The results showed that:①The input of biochar to soil respiration under various temperature conditions was promoted in the early stage and inhibited in the later stage, and after 14 days of cultivation, the input of biochar significantly reduced the temperature sensitivity values of soil respiration. ② For soil organic carbon fractions, the decline rate of the content of soil inert organic carbon under the CK treatment increased with increasing temperature, and was sensitive to the changes in temperature. However, the input of biochar significantly reduced the temperature sensitivity of the soil inert organic carbon, and narrowed its decline rate under each temperature condition. The decline rate did not increase with increasing temperature. ③The 16S rDNA high-throughput sequencing results showed that at the end of the cultivation, the relative abundance of in the soil of the CK treatment decreased with increasing temperature, and the input of biochar significantly increased its temperature sensitivity, making the decline rate increase. In contrast to , the relative abundance of in soil of the CK treatment increased significantly with increasing temperature, and the input of biochar significantly reduced its temperature sensitivity, rendering the difference of relative abundance between each temperature condition not significant. This study shows that the input of biochar can significantly reduce the temperature sensitivity of soil respiration, which is related to decreased temperature sensitivity of soil inert organic carbon, and the change in the relative abundance of and after the input of biochar.
为探究生物炭对土壤呼吸温度敏感性及微生物群落结构的影响，对土壤进行了室内培养试验，设置了两个主要处理，即对照（CK）和添加3%（质量比）生物炭（BC）。每个主要处理包含四个温度条件。分析了土壤CO排放变化、不同土壤有机碳含量以及土壤细菌群落特征。结果表明：①在不同温度条件下，生物炭对土壤呼吸的输入在前期促进，后期抑制，培养14天后，生物炭的输入显著降低了土壤呼吸的温度敏感性值。②对于土壤有机碳组分，CK处理下土壤惰性有机碳含量的下降速率随温度升高而增加，对温度变化敏感。然而，生物炭的输入显著降低了土壤惰性有机碳的温度敏感性，并缩小了其在各温度条件下的下降速率。下降速率并未随温度升高而增加。③16S rDNA高通量测序结果表明，培养结束时，CK处理土壤中 的相对丰度随温度升高而降低，生物炭的输入显著增加了其温度敏感性，使下降速率增加。与之相反，CK处理土壤中 的相对丰度随温度升高显著增加，生物炭的输入显著降低了其温度敏感性，使各温度条件下相对丰度的差异不显著。本研究表明，生物炭的输入可显著降低土壤呼吸的温度敏感性，这与土壤惰性有机碳温度敏感性降低以及生物炭输入后 和 相对丰度的变化有关。
