Faculty of Human Life Science, Osaka City University, Osaka City, Osaka, Japan.
Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima City, Tokushima Japan.
PLoS One. 2020 Nov 2;15(11):e0241290. doi: 10.1371/journal.pone.0241290. eCollection 2020.
Alginetin is the major product formed from pentoses and hexurionic acids. Alginetin is producted by cooking process of food including pection, a naturally-occurring polysacharride found in many plants. However, the biological interaction and toxicity of alginetin are not known at all. The aim of the present study was to investigate the cellular actions of alginetin on rat thymic lymphocytes. The effects of alginetin on the cell were examined using flow cytometry with fluorescent probes. Alginetin increased cellular content of non-protein thiols ([NPT]i) and elevated intracellular Zn2+ levels ([Zn2+]i). Chelation of intracellular Zn2+ reduced the effect of alginetin on [NPT]i, and chelation of external Zn2+ almost completely diminished alginetin-induced elevation of [Zn2+]i, indicating that alginetin treatment increased Zn2+ influx. Increased [NPT]i and [Zn2+]i levels in response to alginetin were positively correlated. Alginetin protected cells against oxidative stress induced by hydrogen peroxide and Ca2+ overload by calcium ionophore. It is considered that the increases in [NPT]i and [Zn2+]i are responsible for the cytoprotective activity of alginetin because NPT attenuates oxidative stress and Zn2+ competes with Ca2+. Alginetin may be produced during manufacturing of jam, which may provide additional health benefits of jam.
藻朊酸是戊糖和己糖二酸形成的主要产物。藻朊酸是由果胶等食物经过烹饪过程产生的，果胶是一种天然存在于许多植物中的多糖。然而，藻朊酸的生物相互作用和毒性还完全不清楚。本研究的目的是研究藻朊酸对大鼠胸腺淋巴细胞的细胞作用。使用带有荧光探针的流式细胞术检查藻朊酸对细胞的影响。藻朊酸增加了非蛋白巯基（[NPT]i）的细胞含量，并升高了细胞内 Zn2+水平（[Zn2+]i）。细胞内 Zn2+螯合减少了藻朊酸对[NPT]i 的作用，而外部 Zn2+螯合几乎完全消除了藻朊酸诱导的[Zn2+]i升高，表明藻朊酸处理增加了 Zn2+内流。响应藻朊酸增加的[NPT]i 和 [Zn2+]i 水平呈正相关。藻朊酸通过过氧化氢和 Ca2+载体引起的 Ca2+过载保护细胞免受氧化应激。认为[NPT]i 和 [Zn2+]i 的增加是藻朊酸细胞保护活性的原因，因为 NPT 减轻了氧化应激，而 Zn2+与 Ca2+竞争。藻朊酸可能在果酱的制造过程中产生，这可能为果酱提供额外的健康益处。
