Radulescu Cristiana, Buruleanu Lavinia Claudia, Nicolescu Cristina Mihaela, Olteanu Radu Lucian, Bumbac Marius, Holban Georgeta Carmen, Simal-Gandara Jesus
Faculty of Sciences and Arts, Valahia University of Targoviste, 130004 Targoviste, Romania.
Institute of Multidisciplinary Research for Science and Technology, Valahia University of Targoviste, 130004 Targoviste, Romania.
Plants (Basel). 2020 Oct 30;9(11):1470. doi: 10.3390/plants9111470.
The therapeutic benefits of extracts obtained from different red grape fractions were thoroughly studied, however, data regarding the comparison of phytochemical extracts prepared from the same varieties coming from organic conventional management systems are rather lacking. The present study aimed at comparing some of the phytochemical characteristics and antimicrobial activity of hydroalcoholic (50% ) extracts obtained from four varieties of red grapes cultivated respectively in organic and conventional vineyards. Total flavonoid content, total phenolic compounds, and antioxidant activity were determined by molecular absorption spectroscopy. Antimicrobial activity of the studied extracts was evaluated against common bacterial strains isolated from different habitats according to specific lab procedures. The analyses were performed in solid broths by applying the disk diffusion method, which allowed for the simultaneous determination of the spectrum of the sensitivity of the tested bacteria as well as the values of the minimum inhibition concentration (MIC). It was found that favorable antagonistic activities against the tested bacteria strains were exhibited by the hydroalcoholic extracts from the seeds of the organic varieties, respectively the skin of the conventional varieties.
人们对从不同红葡萄组分中提取的提取物的治疗益处进行了深入研究，然而，关于来自有机和传统管理系统的同一品种制备的植物化学提取物比较的数据却相当匮乏。本研究旨在比较分别从有机葡萄园和传统葡萄园种植的四个红葡萄品种中获得的水醇（50%）提取物的一些植物化学特性和抗菌活性。通过分子吸收光谱法测定总黄酮含量、总酚化合物和抗氧化活性。根据特定实验室程序，评估所研究提取物对从不同生境分离出的常见细菌菌株的抗菌活性。分析在固体肉汤中采用纸片扩散法进行，该方法可同时测定受试细菌的敏感性谱以及最低抑菌浓度（MIC）值。结果发现，有机品种种子的水醇提取物和传统品种果皮的水醇提取物对受试细菌菌株表现出良好的拮抗活性。
