Topcuoglu Nursen, Ozan Fatih, Ozyurt Mustafa, Kulekci Guven
Department of Microbiology, Faculty of Dentistry, Istanbul University, Istanbul, TURKIYE.
Eur J Dent. 2012 Oct;6(4):428-33.
The aim of this study was to evaluate the antibacterial property of glass-ionomer cement (GIC) containing propolis against Streptococcus mutans and its effect on the in vitro S. mutans biofilm formation.
Ethanolic extract of propolis (EEP) was prepared at two concentrations as 25 and 50%. Three different experimental GIC disks were prepared using pure liquid and liquid solutions diluted with 25 and 50 percent of EEP concentrations. Minimum inhibitory concentration (MIC) of EEP on the growth of S. mutans ATCC 25175 was determined by using agar dilution method. Agar diffusion test and an in vitro S. mutans biofilm assay for GIC disks with and without EEP were performed.
MIC values of Turkish propolis for S. mutans ATCC 25175 was found as 25 μg/mL. Experimental GICs containing propolis exhibited inhibition zones and their dry biofilm weights were less than the pure GIC. The bacterial density was lower in the GIC containing 50% EEP.
A distinct antibacterial and antibiofilm efficacy of propolis containing GIC on S. mutans has been observed. Although further research is needed to show clinical results, antibacterial GIC containing propolis would be a promising material for restoration.
本研究旨在评估含蜂胶的玻璃离子水门汀（GIC）对变形链球菌的抗菌性能及其对体外变形链球菌生物膜形成的影响。
制备两种浓度（25%和50%）的蜂胶乙醇提取物（EEP）。使用纯液体以及用25%和50% EEP浓度稀释的液体溶液制备三种不同的实验性GIC圆盘。采用琼脂稀释法测定EEP对变形链球菌ATCC 25175生长的最低抑菌浓度（MIC）。对含和不含EEP的GIC圆盘进行琼脂扩散试验和体外变形链球菌生物膜测定。
发现土耳其蜂胶对变形链球菌ATCC 25175的MIC值为25μg/mL。含蜂胶的实验性GIC表现出抑菌圈，其干生物膜重量小于纯GIC。含50% EEP的GIC中细菌密度较低。
已观察到含蜂胶的GIC对变形链球菌具有明显的抗菌和抗生物膜功效。尽管需要进一步研究以显示临床结果，但含蜂胶的抗菌GIC将是一种有前景的修复材料。
