Diseases caused by () and spp. can negatively impact turkey farming. The aim of this study was to isolate and characterize multidrug-resistant (MDR) and spp. in healthy and diseased turkeys. A total of 30 fecal samples from healthy turkeys and 25 intestinal samples from diseased turkeys that died of enteritis were collected. Bacterial isolation and identification were based on biochemical properties and polymerase chain reaction (PCR). Antibiogram profiles were determined by disk diffusion. The tetracycline-resistance gene was detected by PCR. All samples were positive for . Only 11 samples (11/30; 36.67%) were positive for spp. from healthy turkeys, whereas 16 (16/25; 64%) samples were positive for spp. from diseased turkeys. isolated from diseased turkeys showed higher resistance to levofloxacin, gentamicin, chloramphenicol, ciprofloxacin, streptomycin, and tetracycline. spp. isolated from healthy turkeys exhibited higher resistance to gentamicin, chloramphenicol, ciprofloxacin, streptomycin, imipenem, and meropenem. All and spp. from both healthy and diseased turkeys were resistant to erythromycin. spp. from both healthy and diseased turkeys were resistant to tetracycline. Multidrug resistance was observed in both and spp. from diseased turkeys. Finally, the gene was detected in 93.1% of the isolates and in 92.59% of the spp. isolates. To the best of our knowledge, this is the first study to isolate and characterize -gene-containing MDR and spp. from healthy and diseased turkeys in Bangladesh. Both microorganisms are of zoonotic significance and represent a significant public health challenge.