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长链非编码RNA ADAMTS9-AS1通过海绵吸附miR-513a-5p抑制乳腺癌细胞的侵袭性特征。

LncRNA ADAMTS9-AS1 Restrains the Aggressive Traits of Breast Carcinoma Cells via Sponging miR-513a-5p.

作者信息

Fang Shiqiang, Zhao Yu, Hu Xiaozhen

机构信息

Department of General Surgery, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, Shandong, People's Republic of China.

Hemodialysis Room, Qingdao Traditional Chinese Medicine Hospital, Qingdao, Shandong, People's Republic of China.

出版信息

Cancer Manag Res. 2020 Oct 28;12:10693-10703. doi: 10.2147/CMAR.S266575. eCollection 2020.

DOI:10.2147/CMAR.S266575
PMID:33149676
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7604470/
Abstract

PURPOSE

Long noncoding RNAs (lncRNAs) exert important functions in the progression of cancers. Currently, we aim to investigate the potential roles of lncRNA ADAM Metallopeptidase with Thrombospondin Type 1 Motif 9 Antisense RNA 1 (ADAMTS9-AS1) in breast carcinoma.

MATERIALS AND METHODS

The expressions of ADAMTS9-AS1 and miR-513a-5p in breast carcinoma tissues and cell lines were detected using qRT-PCR. Cell Counting Kit-8 (CCK-8) and transwell assays were used to assess the viability and invasive ability of breast cancer cells. The direct interaction between ADAMTS9-AS1 and miR-513a-5p was predicted using bioinformatics tools. The target of miR-513a-5p, ZFP36 Ring Finger Protein (ZFP36) was validated by luciferase assay. The expression of ZFP36 was measured using Western blot assay. Breast cancer MDA-MB-231 cells growth in vivo was evaluated using xenograft tumor assay.

RESULTS

ADAMTS9-AS1 was downregulated in breast cancer tissues as well as cell lines. Upregulation of ADAMTS9-AS1 suppressed the growth and invasiveness of breast carcinoma cells in vitro as well as inhibiting cellgrowth in vivo. Furthermore, ZFP36 was manifested as the target gene of miR-513a-5p and negatively modulated by ADAMTS9-AS1. In addition, overexpression of ADAMTS9-AS1 neutralized the promoting impact of miR-513a-5p on the aggressiveness of breast cancer cells.

CONCLUSION

In conclusion, lncRNA ADAMTS9-AS1 inhibited the aggressive phenotypes of breast carcinoma cells via sponging miR-513a-5p and regulating ZFP36.

摘要

目的

长链非编码RNA（lncRNAs）在癌症进展中发挥重要作用。目前，我们旨在研究含血小板反应蛋白基序的金属蛋白酶9反义RNA1（ADAMTS9-AS1）在乳腺癌中的潜在作用。

材料与方法

采用qRT-PCR检测乳腺癌组织和细胞系中ADAMTS9-AS1和miR-513a-5p的表达。使用细胞计数试剂盒-8（CCK-8）和Transwell实验评估乳腺癌细胞的活力和侵袭能力。利用生物信息学工具预测ADAMTS9-AS1与miR-513a-5p之间的直接相互作用。通过荧光素酶报告基因实验验证miR-513a-5p的靶标锌指蛋白36（ZFP36）。使用蛋白质免疫印迹法检测ZFP36的表达。采用异种移植瘤实验评估乳腺癌MDA-MB-231细胞在体内的生长情况。

结果

ADAMTS9-AS1在乳腺癌组织和细胞系中表达下调。上调ADAMTS9-AS1可抑制乳腺癌细胞在体外的生长和侵袭能力，并在体内抑制细胞生长。此外，ZFP36被证明是miR-513a-5p的靶基因，并受到ADAMTS9-AS1的负调控。此外，ADAMTS9-AS1的过表达抵消了miR-513a-5p对乳腺癌细胞侵袭性的促进作用。

结论

总之，lncRNA ADAMTS9-AS1通过吸附miR-513a-5p并调节ZFP36来抑制乳腺癌细胞的侵袭性表型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/c4a0db93ba68/CMAR-12-10693-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/f60034f15429/CMAR-12-10693-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/3b6c65b85f03/CMAR-12-10693-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/8ed2dcdaa2e3/CMAR-12-10693-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/2d311887044f/CMAR-12-10693-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/4361def62cfd/CMAR-12-10693-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/c4a0db93ba68/CMAR-12-10693-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/f60034f15429/CMAR-12-10693-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/3b6c65b85f03/CMAR-12-10693-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/8ed2dcdaa2e3/CMAR-12-10693-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/2d311887044f/CMAR-12-10693-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/4361def62cfd/CMAR-12-10693-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea7c/7604470/c4a0db93ba68/CMAR-12-10693-g0006.jpg

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