Gonzalez María Fernanda, Magdama Freddy, Galarza Luis, Sosa Daynet, Romero Christian
Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Guayaquil, Ecuador.
Facultad de Ingeniería Química, Universidad de Guayaquil, Guayaquil, Ecuador.
Commun Integr Biol. 2020 Oct 20;13(1):160-169. doi: 10.1080/19420889.2020.1829267.
Trichoderma is a saprophytic, soil-borne fungus with a worldwide distribution that has been extensively studied due to their capacity to synthesize secondary metabolites with antimicrobial activity, parasitize other fungi and directly interact with plant roots, inducing resistance to disease and tolerance to abiotic stresses. wilt caused by the soil-inhabiting fungus is considered one of the most important diseases that affect banana cultivars. Currently, more environmentally friendly alternatives to control this disease are being proposed, these strategies include the application of low doses of synthetic fungicides and the use of biocontrol agents such as or . Thus, this study aimed to evaluate under conditions the synergistic effect of the biological control agent C2A combined with low doses of mancozeb to inhibit the mycelial growth of F1. To perform the synergistic essays, 0.1 mg/mL of mancozeb was suspended in PDA plates, then plugs of C2A were placed at the center of the Petri dishes, the plates were incubated for 7 days at 28°C. Results showed that the mycoparasitic capacity of the biocontrol strain to inhibit the mycelial growth of F1 was enhanced approximately 36% compared to the control plates. Although these results are promising, future studies under greenhouse and field conditions are necessary to corroborate the effectiveness of this approach.
木霉是一种腐生的、土壤传播的真菌，在全球范围内均有分布。由于其能够合成具有抗菌活性的次生代谢产物、寄生于其他真菌并直接与植物根系相互作用，诱导植物抗病和耐受非生物胁迫，因此受到了广泛研究。由土壤栖息真菌引起的枯萎病被认为是影响香蕉品种的最重要病害之一。目前，人们正在提出更环保的防治这种病害的替代方法，这些策略包括施用低剂量的合成杀菌剂以及使用生物防治剂，如 或 。因此，本研究旨在评估在 条件下，生物防治剂C2A与低剂量代森锰锌联合使用对抑制F1菌丝生长的协同效应。为了进行协同试验，将0.1 mg/mL的代森锰锌悬浮在PDA平板中，然后将C2A菌块放置在培养皿中心，将平板在28°C下培养7天。结果表明，与对照平板相比，生物防治菌株抑制F1菌丝生长的菌寄生能力提高了约36%。尽管这些结果很有前景，但仍需要在温室和田间条件下进行进一步研究，以证实这种方法的有效性。
