Zhang Fengge, Xu Xixi, Huo Yunqian, Xiao Yan
College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, China.
Front Microbiol. 2019 Jan 7;9:3241. doi: 10.3389/fmicb.2018.03241. eCollection 2018.
spp. are proposed as major plant growth-promoting fungi (PGPF) to increase plants growth and productivity. Mowing can stimulate aboveground regrowth to improve plant biomass and nutritional quality. However, the synergistic effects of and mowing on plants growth, particularly the underlying microbial mechanisms mediated by rhizosphere soil chemical compounds, have rarely been reported. In the present study, we employed T-63 and conducted a pot experiment to investigate the synergistic effect of -inoculation and mowing on alfalfa growth, and the potential soil microbial ecological mechanisms were also explored. Alfalfa treated with inoculation and/or mowing (T, M, and TM) had significant ( < 0.05) increases in plant shoot and root dry weights and soil available nutrients (N, P, and K), compared with those of the control (CK). Non-metric multidimensional scaling (NMDS) demonstrated that the rhizosphere chemical compounds and soil bacterial and fungal communities were, respectively, separated according to different treatments. There was a clear significant ( < 0.05) positive correlation between alfalfa biomass and the relative abundance of ( = 0.3451, = 0.045). However, , and were not significantly correlated with alfalfa biomass. According to structure equation modeling (SEM), abundance and available P served as primary contributors to alfalfa growth promotion. Additionally, -inoculation and mowing altered rhizosphere soil chemical compounds to drive the soil microbial community, indirectly influencing alfalfa growth. Our research provides a basis for promoting alfalfa growth from a soil microbial ecology perspective and may provide a scientific foundation for guiding the farming of alfalfa.
某些物种被提议作为主要的植物促生真菌（PGPF），以促进植物生长和提高生产力。刈割可以刺激地上部分再生长，从而提高植物生物量和营养品质。然而，[具体物种]与刈割对植物生长的协同效应，特别是根际土壤化学化合物介导的潜在微生物机制，鲜有报道。在本研究中，我们采用[具体物种]T - 63进行盆栽试验，以研究接种[具体物种]和刈割对苜蓿生长的协同效应，并探讨潜在的土壤微生物生态机制。与对照（CK）相比，接种和/或刈割处理（T、M和TM）的苜蓿地上部和根部干重以及土壤有效养分（N、P和K）均有显著增加（P < 0.05）。非度量多维尺度分析（NMDS）表明，根际化学化合物以及土壤细菌和真菌群落分别根据不同处理而分离。苜蓿生物量与[具体物种]的相对丰度之间存在明显的显著正相关（P = 0.3451，r = 0.045）。然而，[其他物种]与苜蓿生物量无显著相关性。根据结构方程模型（SEM），[具体物种]丰度和有效磷是促进苜蓿生长的主要因素。此外，接种[具体物种]和刈割改变了根际土壤化学化合物，从而驱动土壤微生物群落，间接影响苜蓿生长。我们的研究从土壤微生物生态学角度为促进苜蓿生长提供了依据，并可能为指导苜蓿种植提供科学基础。
