Žurauskas Mantas, Iyer Rishyashring R, Boppart Stephen A
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Biomed Opt Express. 2021 Jan 22;12(2):981-992. doi: 10.1364/BOE.417183. eCollection 2021 Feb 1.
A new method is presented for full-field optical coherence tomography imaging, which permits capturing single shot phase sensitive imaging through simultaneous acquisition of four phase-shifted images with a single camera using unpolarized light for object illumination. Our method retains the full dynamic range of the camera by using different areas of a single camera sensor to capture each image. We demonstrate the performance of our method by imaging phantoms and live cultures of fibroblast, cancer, and macrophage cells to achieve 59 dB sensitivity with isotropic resolution down to 1 μm, and displacement sensitivity down to 0.1 nm. Our method can serve as a platform for developing high resolution imaging systems because when used in conjunction with broadband spatially incoherent light sources, the resolution is not affected by optical aberrations or speckle noise.
提出了一种用于全场光学相干断层扫描成像的新方法，该方法允许通过使用单台相机同时采集四个相移图像来实现单次相敏成像，使用非偏振光进行物体照明。我们的方法通过使用单个相机传感器的不同区域来捕获每个图像，从而保留了相机的全动态范围。我们通过对成纤维细胞、癌细胞和巨噬细胞的模型和活细胞培养物进行成像，展示了我们方法的性能，实现了59dB的灵敏度，各向同性分辨率低至1μm，位移灵敏度低至0.1nm。我们的方法可以作为开发高分辨率成像系统的平台，因为当与宽带空间非相干光源结合使用时，分辨率不受光学像差或散斑噪声的影响。
