Faculty of Dentistry, the University of Hong Kong, Hong Kong, China.
School of Stomatology, Shenzhen University Health Science Center, Shenzhen, China.
J Dent. 2020 Dec;103:103512. doi: 10.1016/j.jdent.2020.103512. Epub 2020 Nov 4.
To investigate the remineralising and staining effects of sodium fluoride (NaF) with silver nanoparticles (AgNPs) on artificial dentine caries.
Human dentine blocks with artificial caries were divided into four groups. Group 1 received 5 % NaF (22,600 ppm fluoride) with 4000 ppm AgNPs; group 2 received 4000 ppm AgNPs; group 3 received 5 % NaF, group 4 received deionised water (negative control). All groups underwent three biochemical cycles. Each cycle included Streptococcus mutans biofilm challenge and remineralisation process. The lesion depth, mineral-organic content, surface morphology and crystal characteristics of dentine blocks were evaluated using micro-computed tomography, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM) and X-ray diffraction. Colour change of dentine blocks was assessed using spectrophotometry.
The mean lesion depths of groups 1-4 were 151.13 ± 29.13 μm, 172.38 ± 23.44 μm, 190.41 ± 32.81 μm and 221.24 ± 27.91 μm, respectively. The hydrogen phosphate-to-amide I ratios of groups 1-4 were 5.98 ± 0.36, 3.86 ± 0.56, 4.00 ± 0.67 and 2.53 ± 0.40, respectively. There was no significant interaction effect between AgNPs and NaF. SEM showed less exposure of dentine collagen fibres in group 1 when compared to other groups. X-ray diffraction revealed presence of silver chloride and metallic silver in group 1 and 2. There was no significant difference in colour change among the four groups (p = 0.74).
NaF solution with AgNPs can remineralise dentine caries without staining.
Sodium fluoride solutions that include silver nanoparticles have potential uses in the management of caries.
研究纳米银（AgNPs）协同氟化钠（NaF）对人工牙本质龋再矿化和染色的影响。
将带有人工龋的人牙本质块分为四组。第 1 组接受 5%NaF（22600ppm 氟化物）和 4000ppm AgNPs；第 2 组接受 4000ppm AgNPs；第 3 组接受 5%NaF；第 4 组接受去离子水（阴性对照）。所有组均经历三个生物化学循环。每个循环包括变形链球菌生物膜挑战和再矿化过程。使用微计算机断层扫描、傅里叶变换红外光谱、扫描电子显微镜（SEM）和 X 射线衍射评估牙本质块的病变深度、矿化-有机质含量、表面形态和晶体特征。使用分光光度法评估牙本质块的颜色变化。
第 1-4 组的平均病变深度分别为 151.13±29.13μm、172.38±23.44μm、190.41±32.81μm 和 221.24±27.91μm。第 1-4 组的磷酸氢盐与酰胺 I 比值分别为 5.98±0.36、3.86±0.56、4.00±0.67 和 2.53±0.40。AgNPs 和 NaF 之间没有显著的相互作用效应。与其他组相比，SEM 显示第 1 组牙本质胶原纤维暴露较少。X 射线衍射显示第 1 组和第 2 组存在氯化银和金属银。四组之间的颜色变化无显著差异（p=0.74）。
含 AgNPs 的 NaF 溶液可使牙本质龋再矿化而不染色。
含纳米银的氟化钠溶液在龋病管理中有潜在用途。
