Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Department of Oral Medicine, Shanxi Provincial People's Hospital, Taiyuan, Shanxi, China.
Clin Oral Investig. 2022 Mar;26(3):2441-2451. doi: 10.1007/s00784-021-04210-1. Epub 2021 Oct 12.
To investigate the antibiofilm and remineralising effects of the dual-action peptide GA-KR12 on artificial enamel caries.
Enamel blocks with artificial caries were treated with sterilised deionised water as control or GA-KR12. The blocks underwent biochemical cycling with Streptococcus mutans for 3 weeks. The architecture, viability, and growth kinetics of the biofilm were determined, respectively, by scanning electron microscopy (SEM), confocal laser scanning microscopy, and quantitative (culture colony-forming units, CFUs). The mineral loss, calcium-to-phosphorus ratio, surface morphology, and crystal characteristics of the enamel surface were determined, respectively, using micro-computed tomography, energy dispersive spectroscopy, SEM, and X-ray diffraction (XRD).
SEM showed confluent growth of S. mutans in the control group but not in the GA-KR12-treated group. The dead-to-live ratios of the control and GA-KR12-treated groups were 0.42 ± 0.05 and 0.81 ± 0.08, respectively (p < 0.001). The log CFUs of the control and GA-KR12-treated groups were 8.15 ± 0.32 and 6.70 ± 0.49, respectively (p < 0.001). The mineral losses of the control and GA-KR12-treated groups were 1.39 ± 0.09 gcm and 1.19 ± 0.05 gcm, respectively (p < 0.001). The calcium-to-phosphorus molar ratios of the control and GA-KR12-treated groups were 1.47 ± 0.03 and 1.57 ± 0.02, respectively (p < 0.001). A uniformly remineralised prismatic pattern on enamel blocks was observed in the GA-KR12-treated but not in the control group. The hydroxyapatite in the GA-KR12-treated group was better crystallised than that in the control group.
The dual-action peptide GA-KR12 inhibited the growth of S. mutans biofilm and promoted the remineralisation of enamel caries.
GA-KR12 potentially is applicable for managing enamel caries.
研究双功能肽 GA-KR12 对人工釉质龋的抗生物膜和再矿化作用。
用灭菌去离子水（对照）或 GA-KR12 处理有龋人工釉质块。将块体用变异链球菌进行生物化学循环 3 周。分别通过扫描电子显微镜（SEM）、共聚焦激光扫描显微镜和定量（培养菌落形成单位，CFU）来确定生物膜的结构、活力和生长动力学。分别通过微计算机断层扫描、能量色散光谱、SEM 和 X 射线衍射（XRD）来确定釉质表面的矿物质损失、钙磷比、表面形貌和晶体特征。
SEM 显示对照组中变异链球菌呈融合生长，而 GA-KR12 处理组则无。对照组和 GA-KR12 处理组的死/活比分别为 0.42±0.05 和 0.81±0.08（p<0.001）。对照组和 GA-KR12 处理组的 log CFU 分别为 8.15±0.32 和 6.70±0.49（p<0.001）。对照组和 GA-KR12 处理组的矿物质损失分别为 1.39±0.09 gcm 和 1.19±0.05 gcm（p<0.001）。对照组和 GA-KR12 处理组的钙磷摩尔比分别为 1.47±0.03 和 1.57±0.02（p<0.001）。在 GA-KR12 处理组中观察到釉质块上有均匀的再矿化棱柱图案，而在对照组中则没有。GA-KR12 处理组的羟基磷灰石结晶性优于对照组。
双功能肽 GA-KR12 抑制变异链球菌生物膜的生长并促进釉质龋的再矿化。
GA-KR12 可能适用于管理釉质龋。
