Laboratório de Biotecnologia de Polímeros Naturais - BIOPOL, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, 59.078-970, Brazil.
Programa de Pós-graduação em Ciências da Saúde, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59012-570, Brazil.
Mar Drugs. 2020 Apr 29;18(5):234. doi: 10.3390/md18050234.
Sulfated polysaccharides (SPs) obtained from green seaweeds are structurally heterogeneous molecules with multifunctional bioactivities. In this work, two sulfated and pyruvated galactans were purified from var. (named SP1 and SP2), and their immunostimulatory effect was evaluated using cultured murine macrophage cells. Both SPs equally increased the production of nitric oxide, reactive oxygen species, and the proinflammatory cytokines TNF-α and IL-6. NMR spectroscopy revealed that both galactans were composed primarily of 3)-β-d-Galp-(1→3) units. Pyruvate groups were also found, forming five-membered cyclic ketals as 4,6--(1'carboxy)-ethylidene-β-d-Galp residues. Some galactoses are sulfated at C-2. In addition, only SP2 showed some galactose units sulfated at C-4, indicating that sulfation at this position is not essential for the immunomodulatory activity of these galactans. Overall, the data showed that the galactans of exhibited immunostimulating activity with potential therapeutic applications, which can be used in the development of new biomedical products.
从绿藻中提取的硫酸化多糖（SPs）是具有多功能生物活性的结构不均一的分子。在这项工作中，从 var. 中纯化得到了两种硫酸化和丙酮酸化的半乳糖聚糖（分别命名为 SP1 和 SP2），并使用培养的鼠巨噬细胞评价了它们的免疫刺激作用。这两种多糖均能同等程度地增加一氧化氮、活性氧和促炎细胞因子 TNF-α 和 IL-6 的产生。NMR 光谱表明，两种半乳糖聚糖均主要由 3）-β-d-Galp-(1→3)单元组成。还发现了丙酮酸基团，形成作为 4,6--(1'羧基）-乙基二亚甲基-β-d-Galp 残基的五元环缩酮。一些半乳糖在 C-2 位被硫酸化。此外，只有 SP2 显示出一些在 C-4 位硫酸化的半乳糖单元，表明该位置的硫酸化对于这些半乳糖聚糖的免疫调节活性不是必需的。总体而言，数据表明 var. 的半乳糖聚糖具有免疫刺激活性，具有潜在的治疗应用，可用于开发新的生物医学产品。
