• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

癌症改变细胞外囊泡的代谢指纹图谱。

Cancer Alters the Metabolic Fingerprint of Extracellular Vesicles.

作者信息

Palviainen Mari, Laukkanen Kirsi, Tavukcuoglu Zeynep, Velagapudi Vidya, Kärkkäinen Olli, Hanhineva Kati, Auriola Seppo, Ranki Annamari, Siljander Pia

机构信息

EV group, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, 00790 Helsinki, Finland.

CURED, Drug Research Program, Faculty of Pharmacy, Division of Pharmaceutical Biosciences, University of Helsinki, 00790 Helsinki, Finland.

出版信息

Cancers (Basel). 2020 Nov 6;12(11):3292. doi: 10.3390/cancers12113292.

DOI:10.3390/cancers12113292
PMID:33172086
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7694806/
Abstract

Cancer alters cell metabolism. How these changes are manifested in the metabolite cargo of cancer-derived extracellular vesicles (EVs) remains poorly understood. To explore these changes, EVs from prostate, cutaneous T-cell lymphoma (CTCL), colon cancer cell lines, and control EVs from their noncancerous counterparts were isolated by differential ultracentrifugation and analyzed by nanoparticle tracking analysis (NTA), electron microscopy (EM), Western blotting, and liquid chromatography-mass spectrometry (LC-MS). Although minor differences between the cancerous and non-cancerous cell-derived EVs were observed by NTA and Western blotting, the largest differences were detected in their metabolite cargo. Compared to EVs from noncancerous cells, cancer EVs contained elevated levels of soluble metabolites, e.g., amino acids and B vitamins. Two metabolites, proline and succinate, were elevated in the EV samples of all three cancer types. In addition, folate and creatinine were elevated in the EVs from prostate and CTCL cancer cell lines. In conclusion, we present the first evidence in vitro that the altered metabolism of different cancer cells is reflected in common metabolite changes in their EVs. These results warrant further studies on the significance and usability of this metabolic fingerprint in cancer.

摘要

癌症会改变细胞代谢。这些变化如何在癌症来源的细胞外囊泡（EVs）的代谢物货物中体现，目前仍知之甚少。为了探究这些变化，通过差速超速离心法从前列腺、皮肤T细胞淋巴瘤（CTCL）、结肠癌细胞系中分离出EVs，并从其对应的非癌细胞中分离出对照EVs，然后通过纳米颗粒跟踪分析（NTA）、电子显微镜（EM）、蛋白质免疫印迹法和液相色谱 - 质谱联用（LC - MS）进行分析。尽管通过NTA和蛋白质免疫印迹法观察到癌源性和非癌源性细胞衍生的EVs之间存在细微差异，但在它们的代谢物货物中检测到的差异最为显著。与来自非癌细胞的EVs相比，癌症EVs中可溶性代谢物（如氨基酸和B族维生素）的水平升高。在所有三种癌症类型的EV样本中，脯氨酸和琥珀酸这两种代谢物的含量都有所升高。此外，前列腺和CTCL癌细胞系来源的EVs中叶酸和肌酐含量升高。总之，我们首次在体外证明，不同癌细胞代谢的改变反映在其EVs中常见的代谢物变化上。这些结果值得进一步研究这种代谢指纹在癌症中的意义和实用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/6051b9cfe5ef/cancers-12-03292-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/c757d65ed0a7/cancers-12-03292-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/0787993b9767/cancers-12-03292-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/633b963f9434/cancers-12-03292-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/764173bbb146/cancers-12-03292-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/19c6ee6f558f/cancers-12-03292-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/be2addcff5f8/cancers-12-03292-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/6051b9cfe5ef/cancers-12-03292-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/c757d65ed0a7/cancers-12-03292-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/0787993b9767/cancers-12-03292-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/633b963f9434/cancers-12-03292-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/764173bbb146/cancers-12-03292-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/19c6ee6f558f/cancers-12-03292-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/be2addcff5f8/cancers-12-03292-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c95/7694806/6051b9cfe5ef/cancers-12-03292-g007.jpg

相似文献

1
Cancer Alters the Metabolic Fingerprint of Extracellular Vesicles.癌症改变细胞外囊泡的代谢指纹图谱。
Cancers (Basel). 2020 Nov 6;12(11):3292. doi: 10.3390/cancers12113292.
2
Metabolomic Profiling of Extracellular Vesicles and Alternative Normalization Methods Reveal Enriched Metabolites and Strategies to Study Prostate Cancer-Related Changes.细胞外囊泡代谢组学分析及替代标准化方法揭示丰富的代谢物，并为研究前列腺癌相关变化提供策略。
Theranostics. 2017 Aug 23;7(16):3824-3841. doi: 10.7150/thno.19890. eCollection 2017.
3
Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research.比较小型尿液细胞外囊泡纯化方法以进行RNA测序——助力稳健且无创的生物标志物研究。
Biomol Detect Quantif. 2019 Jun 4;17:100089. doi: 10.1016/j.bdq.2019.100089. eCollection 2019 Mar.
4
Metabolic signature of extracellular vesicles depends on the cell culture conditions.细胞外囊泡的代谢特征取决于细胞培养条件。
J Extracell Vesicles. 2019 Apr 4;8(1):1596669. doi: 10.1080/20013078.2019.1596669. eCollection 2019.
5
Investigation of procoagulant activity in extracellular vesicles isolated by differential ultracentrifugation.通过差速超速离心法分离的细胞外囊泡中促凝血活性的研究。
J Extracell Vesicles. 2018 Mar 26;7(1):1454777. doi: 10.1080/20013078.2018.1454777. eCollection 2018.
6
Preliminary investigation of extracellular vesicles in mammary cancer of dogs and cats: Identification and characterization.犬猫乳腺癌细胞外囊泡的初步研究：鉴定与表征
Vet Comp Oncol. 2018 Dec;16(4):489-496. doi: 10.1111/vco.12405. Epub 2018 May 30.
7
Proteomic characterization of macro-, micro- and nano-extracellular vesicles derived from the same first trimester placenta: relevance for feto-maternal communication.从同一妊娠早期胎盘来源的大、小、纳米细胞外囊泡中进行蛋白质组学表征：与胎母通讯的相关性。
Hum Reprod. 2016 Apr;31(4):687-99. doi: 10.1093/humrep/dew004. Epub 2016 Feb 1.
8
Chromatography and its hyphenation to mass spectrometry for extracellular vesicle analysis.用于细胞外囊泡分析的色谱及其与质谱联用技术。
J Chromatogr A. 2016 Mar 25;1439:26-41. doi: 10.1016/j.chroma.2016.01.017. Epub 2016 Jan 11.
9
Isolation and Characterization of Cetacean Cell-Derived Extracellular Vesicles.鲸类细胞衍生细胞外囊泡的分离与鉴定
Animals (Basel). 2023 Oct 24;13(21):3304. doi: 10.3390/ani13213304.
10
Metabolic alterations in urine extracellular vesicles are associated to prostate cancer pathogenesis and progression.尿液细胞外囊泡中的代谢改变与前列腺癌的发病机制和进展相关。
J Extracell Vesicles. 2018 May 7;7(1):1470442. doi: 10.1080/20013078.2018.1470442. eCollection 2018.

引用本文的文献

1
Extracellular Vesicles Derived from Breast Cancer Cells: Emerging Biomarkers of Tumor Progression and Metastasis.源自乳腺癌细胞的细胞外囊泡：肿瘤进展和转移的新兴生物标志物
Biomolecules. 2025 Aug 19;15(8):1195. doi: 10.3390/biom15081195.
2
Extracellular vesicles: from intracellular trafficking molecules to fully fortified delivery vehicles for cancer therapeutics.细胞外囊泡：从细胞内运输分子到用于癌症治疗的全面强化递送载体。
Nanoscale Adv. 2025 Jan 15;7(4):934-962. doi: 10.1039/d4na00393d. eCollection 2025 Feb 11.
3
Metabolic features of tumor-derived extracellular vesicles: challenges and opportunities.

本文引用的文献

1
Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo-Implications for biomarker discovery.人血浆和血清中的细胞外囊泡是细胞外囊泡货物的载体——对生物标志物发现的启示。
PLoS One. 2020 Aug 19;15(8):e0236439. doi: 10.1371/journal.pone.0236439. eCollection 2020.
2
Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers.细胞外囊泡和颗粒生物标志物可定义多种人类癌症。
Cell. 2020 Aug 20;182(4):1044-1061.e18. doi: 10.1016/j.cell.2020.07.009. Epub 2020 Aug 13.
3
Lipid metabolism alteration contributes to and maintains the properties of cancer stem cells.
肿瘤来源细胞外囊泡的代谢特征：挑战与机遇
Extracell Vesicles Circ Nucl Acids. 2024 Aug 27;5(3):455-470. doi: 10.20517/evcna.2024.12. eCollection 2024.
4
The relationship between immune cells and prostate cancer, and the mediating role of metabolites: a Mendelian randomization study.免疫细胞与前列腺癌的关系，及其代谢物的中介作用：一项孟德尔随机化研究。
Sci Rep. 2024 Oct 31;14(1):26217. doi: 10.1038/s41598-024-78085-z.
5
Transcending frontiers in prostate cancer: the role of oncometabolites on epigenetic regulation, CSCs, and tumor microenvironment to identify new therapeutic strategies.超越前列腺癌的前沿：代谢物在表观遗传调控、CSCs 和肿瘤微环境中的作用，以确定新的治疗策略。
Cell Commun Signal. 2024 Jan 12;22(1):36. doi: 10.1186/s12964-023-01462-0.
6
Prostate cancer-derived small extracellular vesicle proteins: the hope in diagnosis, prognosis, and therapeutics.前列腺癌来源的小细胞外囊泡蛋白：在诊断、预后和治疗中的希望。
J Nanobiotechnology. 2023 Dec 14;21(1):480. doi: 10.1186/s12951-023-02219-0.
7
Mendelian randomization analysis to elucidate the causal relationship between small molecule metabolites and ovarian cancer risk.孟德尔随机化分析以阐明小分子代谢物与卵巢癌风险之间的因果关系。
Front Oncol. 2023 Nov 28;13:1291033. doi: 10.3389/fonc.2023.1291033. eCollection 2023.
8
Metabolomics of Extracellular Vesicles: A Future Promise of Multiple Clinical Applications.细胞外囊泡的代谢组学：多种临床应用的未来前景。
Int J Nanomedicine. 2022 Dec 7;17:6113-6129. doi: 10.2147/IJN.S390378. eCollection 2022.
9
A quick pipeline for the isolation of 3D cell culture-derived extracellular vesicles.一种快速分离 3D 细胞培养衍生细胞外囊泡的管道。
J Extracell Vesicles. 2022 Oct;11(10):e12273. doi: 10.1002/jev2.12273.
10
Integrative Analysis of Bulk RNA-Seq and Single-Cell RNA-Seq Unveils the Characteristics of the Immune Microenvironment and Prognosis Signature in Prostate Cancer.批量RNA测序和单细胞RNA测序的综合分析揭示了前列腺癌免疫微环境的特征和预后特征。
J Oncol. 2022 Jul 19;2022:6768139. doi: 10.1155/2022/6768139. eCollection 2022.
脂质代谢改变有助于并维持癌症干细胞的特性。
Theranostics. 2020 May 30;10(16):7053-7069. doi: 10.7150/thno.41388. eCollection 2020.
4
Proline Metabolism in Tumor Growth and Metastatic Progression.脯氨酸代谢在肿瘤生长和转移进展中的作用
Front Oncol. 2020 May 15;10:776. doi: 10.3389/fonc.2020.00776. eCollection 2020.
5
New findings on urinary prostate cancer metabolome through combined GC-MS and H NMR analytical platforms.通过气相色谱 - 质谱联用（GC-MS）和核磁共振氢谱（H NMR）分析平台对前列腺癌尿液代谢组的新发现。
Metabolomics. 2020 Jun 3;16(6):70. doi: 10.1007/s11306-020-01691-1.
6
Creatine metabolism: energy homeostasis, immunity and cancer biology.肌酸代谢：能量平衡、免疫和癌症生物学。
Nat Rev Endocrinol. 2020 Aug;16(8):421-436. doi: 10.1038/s41574-020-0365-5. Epub 2020 Jun 3.
7
Exploiting the folate receptor α in oncology.在肿瘤学中利用叶酸受体α。
Nat Rev Clin Oncol. 2020 Jun;17(6):349-359. doi: 10.1038/s41571-020-0339-5. Epub 2020 Mar 9.
8
Understanding the role of key amino acids in regulation of proline dehydrogenase/proline oxidase (prodh/pox)-dependent apoptosis/autophagy as an approach to targeted cancer therapy.理解关键氨基酸在脯氨酸脱氢酶/脯氨酸氧化酶（prodh/pox）依赖性细胞凋亡/自噬调控中的作用，作为一种靶向癌症治疗的方法。
Mol Cell Biochem. 2020 Mar;466(1-2):35-44. doi: 10.1007/s11010-020-03685-y. Epub 2020 Jan 13.
9
Metabolism and cancer: the future is now.代谢与癌症：现在即是未来。
Br J Cancer. 2020 Jan;122(2):133-135. doi: 10.1038/s41416-019-0667-3. Epub 2019 Dec 10.
10
Molecular pathogenesis of tumorigenesis caused by succinate dehydrogenase defect.琥珀酸脱氢酶缺陷导致肿瘤发生的分子发病机制。
Eur J Cell Biol. 2020 Jan;99(1):151057. doi: 10.1016/j.ejcb.2019.151057. Epub 2019 Nov 15.