以及巴西东北部性活跃女性中它与其他性传播合并感染的关联。
and Its Association with Other Sexually Transmitted Coinfection among Sexually Active Women from the Northeast of Brazil.
作者信息
Paula Almeida Cunha Ana, Kassandra Pereira Belfort Ilka, Pedro Belfort Mendes Francisco, Rodrigues Bastos Dos Santos Gerusinete, Henrique de Lima Costa Lucas, de Matos Monteiro Pablo, Lemos Gaspar Renata, Borges Ferreira Mariele, de Sá Ferreira Alice, Cristina Moutinho Monteiro Sally, Castello Branco Vidal Flávia
机构信息
Federal University of Maranhão, São Luís, Brazil.
Department of Pharmacy, Federal University of Maranhão, São Luís, Brazil.
出版信息
Interdiscip Perspect Infect Dis. 2020 Oct 29;2020:8838317. doi: 10.1155/2020/8838317. eCollection 2020.
OBJECTIVE
To verify the association between HPV infection and the presence of coinfections (, , and ) in women in the state of Maranhão.
METHODS
HPV-DNA detection was performed by the nested PCR, using the primers PGMY09/11 and GP + 5/GP + 6. For the identification of sexually transmitted agents, conventional PCR was performed using the following primers: KL1/KL2 (), TVA5/TVA6 (), and HO1/HO3 (). DNA-HPV positive samples were subjected to automated sequencing for genotyping.
RESULTS
Among the 353 women evaluated, 204 (57.8%) had HPV-DNA, of which 140 (68.6%) exhibited HPV/STIs, while 64 (31.4%) had the only HPV. infection showed a positive association with HPV (=0.003). Women without cervical lesions were predominant (327/92.6%); however, the largest number of lesions was reported in women who had HPV/coinfections (18/8.8%). Multiple regression analysis showed that both HPV only and the concomitant presence of HPV/STI were able to indicate the occurrence of epithelial lesions ( = 0.164; 2 = 0.027).
CONCLUSION
The findings suggest that the presence of can contribute to HPV infection, and HPV/IST association may influence the development of cervical intraepithelial lesions that are precursors of cervical cancer.
目的
验证马拉尼昂州女性人乳头瘤病毒（HPV）感染与合并感染（、和）之间的关联。
方法
采用巢式聚合酶链反应（PCR），使用引物PGMY09/11和GP + 5/GP + 6进行HPV-DNA检测。为鉴定性传播病原体，使用以下引物进行常规PCR：KL1/KL2（）、TVA5/TVA6（）和HO1/HO3（）。HPV-DNA阳性样本进行自动测序以进行基因分型。
结果
在评估的353名女性中，204名（57.8%）检测出HPV-DNA，其中140名（68.6%）表现为HPV/性传播感染（STIs）合并感染，而64名（31.4%）仅感染HPV。感染与HPV呈正相关（=0.003）。无宫颈病变的女性占多数（327/92.6%）；然而，HPV/合并感染的女性中报告的病变数量最多（18/8.8%）。多元回归分析表明，仅HPV感染以及HPV/STI合并感染均能够提示上皮病变的发生（=0.164；2 = 0.027）。
结论
研究结果表明，的存在可能促成HPV感染，且HPV/STI合并感染可能影响宫颈癌前体宫颈上皮内病变的发展。
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