Food Research Institute, National Food and Agriculture Research Organization, 2-1-12 Kan-nondai, Tsukuba, 305-8642, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Food Research Institute, National Food and Agriculture Research Organization, 2-1-12 Kan-nondai, Tsukuba, 305-8642, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Anal Biochem. 2020 Dec 15;611:114018. doi: 10.1016/j.ab.2020.114018. Epub 2020 Nov 10.
Advanced glycation end products (AGEs) are a heterogenous group of glycation adducts on amino acids produced with sugars or dicarbonyls. Intracellular inflammation triggered by binding of AGEs to receptor for AGEs (RAGE) is linked to some chronic diseases. Here, we established a competitive assay format to comprehensively quantify AGEs which bound to RAGE. RAGE-binding activities of sugar- and dicarbonyl-derived AGEs were correlated with oxidative stress in cultured cells generated by the respective AGEs, suggesting that this would be a promising method for evaluating AGEs which could affect cellular functions despite limited information on individual glycation adducts.
糖基化终产物（AGEs）是由糖或二羰基化合物与氨基酸发生糖化反应而产生的一组不均一的糖化加合物。AGEs 与 AGEs 受体（RAGE）结合引发的细胞内炎症与一些慢性疾病有关。在这里，我们建立了一种竞争测定方法，以全面定量与 RAGE 结合的 AGEs。糖和二羰基衍生的 AGEs 与各自的 AGEs 在培养细胞中产生的氧化应激相关的 RAGE 结合活性表明，这将是一种有前途的方法，可用于评估可能影响细胞功能的 AGEs，尽管对单个糖化加合物的信息有限。
