RIKEN Center for Integrative Medical Sciences, Kanagawa, Japan.
Osaka City University Graduate School of Medicine, Osaka, Japan.
Sci Rep. 2020 Nov 16;10(1):19933. doi: 10.1038/s41598-020-77105-y.
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex and debilitating disease with no molecular diagnostics and no treatment options. To identify potential markers of this illness, we profiled 48 patients and 52 controls for standard laboratory tests, plasma metabolomics, blood immuno-phenotyping and transcriptomics, and fecal microbiome analysis. Here, we identified a set of 26 potential molecular markers that distinguished ME/CFS patients from healthy controls. Monocyte number, microbiome abundance, and lipoprotein profiles appeared to be the most informative markers. When we correlated these molecular changes to sleep and cognitive measurements of fatigue, we found that lipoprotein and microbiome profiles most closely correlated with sleep disruption while a different set of markers correlated with a cognitive parameter. Sleep, lipoprotein, and microbiome changes occur early during the course of illness suggesting that these markers can be examined in a larger cohort for potential biomarker application. Our study points to a cluster of sleep-related molecular changes as a prominent feature of ME/CFS in our Japanese cohort.
肌痛性脑脊髓炎/慢性疲劳综合征（ME/CFS）是一种复杂且使人虚弱的疾病，目前尚无分子诊断方法，也没有治疗选择。为了确定这种疾病的潜在标志物，我们对 48 名患者和 52 名对照者进行了标准实验室检测、血浆代谢组学、血液免疫表型和转录组学以及粪便微生物组分析。在这里，我们确定了一组 26 种潜在的分子标志物，可以将 ME/CFS 患者与健康对照者区分开来。单核细胞数量、微生物组丰度和脂蛋白谱似乎是最具信息量的标志物。当我们将这些分子变化与疲劳的睡眠和认知测量相关联时，我们发现脂蛋白和微生物组谱与睡眠中断最密切相关，而另一组标志物与认知参数相关。在疾病的早期就会出现睡眠、脂蛋白和微生物组的变化，这表明可以在更大的队列中检查这些标志物，以寻找潜在的生物标志物应用。我们的研究表明，与睡眠相关的分子变化群是日本队列中 ME/CFS 的一个突出特征。
