Xu Keqiang, Wu Xiaqing, Cheng Yan, Yan Jiao, Feng Yanlin, Chen Rui, Zheng Runxiao, Li Xi, Song Panpan, Wang Yanjing, Zhang Haiyuan
Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, Jilin, China.
Nanoscale. 2020 Nov 26;12(45):23159-23165. doi: 10.1039/d0nr05097k.
Photothermal therapy (PTT) and chemodynamic therapy (CDT) are promising therapeutic modalities with high specificity, however, a single therapeutic modality cannot maximize therapeutic efficacy. In the present study, glucose oxidase (GOx) was decorated on N-doped carbon (NC) nanoparticles (NPs) as a biomimetic nanoenzyme (NC@GOx NPs), which could promote starvation therapy enhanced PTT and CDT against tumors. GOx could decompose to cut off the supply of energy and nutrients, inducing starvation therapy, which further lowered adenosine triphosphate (ATP) levels, inducing downregulated heat shock proteins and creating a more suitable microenvironment for improving PTT efficacy. Meanwhile, the generated endogenous hydrogen peroxide (H2O2) could be converted into hydroxyl radicals to attack cancer cells. In fact, in vitro and in vivo experiments demonstrated that NC@GOx NPs could effectively kill cancer cells and eliminate tumors. This design provides a strategy for synergistic cancer therapy by using biomimetic nanoenzymes.
光热疗法（PTT）和化学动力疗法（CDT）是具有高特异性的很有前景的治疗方式，然而，单一的治疗方式无法使治疗效果最大化。在本研究中，葡萄糖氧化酶（GOx）被修饰在氮掺杂碳（NC）纳米颗粒（NPs）上作为一种仿生纳米酶（NC@GOx NPs），其可促进饥饿疗法增强对肿瘤的PTT和CDT。GOx可分解以切断能量和营养物质的供应，诱导饥饿疗法，这进一步降低了三磷酸腺苷（ATP）水平，诱导热休克蛋白下调，并为提高PTT疗效创造了更合适的微环境。同时，产生的内源性过氧化氢（H2O2）可转化为羟基自由基以攻击癌细胞。事实上，体外和体内实验表明，NC@GOx NPs可有效杀死癌细胞并消除肿瘤。该设计提供了一种利用仿生纳米酶进行协同癌症治疗的策略。
