Department of Surgery, Faculty of Veterinary Medicine, Alexandria University, Alexandria, 21944, Egypt.
Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi, 441-8580, Japan.
Biol Trace Elem Res. 2021 Oct;199(10):3688-3699. doi: 10.1007/s12011-020-02489-x. Epub 2020 Nov 16.
Treatment of burn wounds has many requirements to ensure wound closure with healthy tissue, increased vascularization, guarantee edema resolution, and control bacterial infection. We propose that titanium oxide (TiO) nanoparticles (NPs) will be more efficient than silver dioxide (AgO) in the treatment of burn wounds. Herein, gelatin loaded NPs (GLT-NPs) were evaluated for their efficacy to regenerate second-degree burn wound in rabbit skin. TEM results revealed that the average particle sizes were ⁓ 7.5 and 17 nm for AgO and TiO NPs, respectively. The results of the in vivo application of GLT-NPs on burn wound in the rabbit revealed that both AgO and TiO NPs were efficient than the control none treated (CTRL) and GLT group. In terms of the healing rate, the GLT-TiO did not show any significant difference than GLT-AgO (99.57% vs. 99.85%, p = 0.2). Meanwhile, the healing rate was significantly higher in both NPs' treated groups than CTRL (94.16%, p < 0.01) and GLT group (95.07%, p < 0.05). Also, the histological analysis using H&E staining showed re-epithelization, less edema, and enhanced vascularization in both GLT-NPs than CTRL and GLT groups. Furthermore, immunohistochemical analysis of TGF-β1 and α-SMA revealed significantly a higher expression in both GLT-NPs groups than CTRL and GLT groups at weeks 1 and 2 (p < 0.05). Interestingly, TGF-β1 and α-SMA were substantially higher in GLT- TiO than GLT-AgO at weeks 1 and 2 (p < 0.05), but the expression was not significant at week 3. In conclusion, GLT-NPs showed higher regenerative capacity and enhanced the healing quality after burn wound compared to CTRL and GLT. Graphical abstract.
治疗烧伤创面有许多要求，以确保用健康组织闭合伤口、增加血管化、保证水肿消退和控制细菌感染。我们提出，氧化钛（TiO）纳米颗粒（NPs）将比氧化银（AgO）在治疗烧伤创面更有效。在此，评估了载明胶的 NPs（GLT-NPs）在兔皮二度烧伤创面再生中的疗效。TEM 结果表明，AgO 和 TiO NPs 的平均粒径分别为 ⁓ 7.5 和 17nm。GLT-NPs 在兔烧伤创面体内应用的结果表明，AgO 和 TiO NPs 均优于未处理的对照（CTRL）和 GLT 组。就愈合率而言，GLT-TiO 与 GLT-AgO 之间无显著差异（99.57%比 99.85%，p=0.2）。同时，与 CTRL 组（94.16%，p<0.01）和 GLT 组（95.07%，p<0.05）相比，两种 NPs 处理组的愈合率均显著更高。此外，H&E 染色的组织学分析显示，与 CTRL 和 GLT 组相比，GLT-NPs 组具有更好的再上皮化、更少的水肿和增强的血管化。此外，TGF-β1 和 α-SMA 的免疫组化分析显示，在第 1 周和第 2 周，GLT-NPs 组的 TGF-β1 和 α-SMA 表达均显著高于 CTRL 和 GLT 组（p<0.05）。有趣的是，在第 1 周和第 2 周，GLT-TiO 组的 TGF-β1 和 α-SMA 表达明显高于 GLT-AgO 组（p<0.05），但第 3 周时差异不显著。总之，与 CTRL 和 GLT 相比，GLT-NPs 显示出更高的再生能力和增强的烧伤创面愈合质量。
