用于检测沙鼠源性汉坦病毒感染的血清学方法：Thottapalayam、Seewis、阿尔泰山和Asama 病毒。

Serological methods for detection of infection with shrew-borne hantaviruses: Thottapalayam, Seewis, Altai, and Asama viruses.

机构信息

Graduate School of Infectious Diseases, Hokkaido University, Sapporo, 060-8638, Japan.

Department of Microbiology and Immunology, Faculty of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.

出版信息

Arch Virol. 2021 Jan;166(1):275-280. doi: 10.1007/s00705-020-04873-3. Epub 2020 Nov 17.

DOI:10.1007/s00705-020-04873-3

PMID:33201342

Abstract

The infectivity of shrew-borne hantaviruses to humans is still unclear because of the lack of a serodiagnosis method for these viruses. In this study, we prepared recombinant nucleocapsid (rN) proteins of Seewis orthohantavirus, Altai orthohantavirus (ALTV), Thottapalayam thottimvirus (TPMV), and Asama orthohantavirus. Using monospecific rabbit sera, no antigenic cross-reactivity was observed. In a serosurvey of 104 samples from renal patients and 271 samples from heathy controls from Sri Lanka, one patient serum and two healthy control sera reacted with rN proteins of ALTV and TPMV, respectively. The novel assays should be applied to investigate potential infectivity of shrew-borne hantaviruses to humans.

摘要

由于缺乏针对这些病毒的血清学诊断方法，因此尚不清楚沙鼠携带的汉坦病毒对人类的传染性。在这项研究中，我们制备了 Seewis 正粘病毒、阿尔泰山正粘病毒（ALTV）、Thottapalayam 托特病毒（TPMV）和 Asama 正粘病毒的重组核衣壳（rN）蛋白。使用单特异性兔血清，未观察到抗原交叉反应。在对来自斯里兰卡的 104 例肾病患者和 271 例健康对照者的血清进行血清学调查中，1 例患者血清和 2 例健康对照者血清分别与 ALTV 和 TPMV 的 rN 蛋白发生反应。这些新的检测方法应可用于研究沙鼠携带的汉坦病毒对人类的潜在感染性。