CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisboa, Portugal.
LEAF - Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa, Portugal.
J Anim Physiol Anim Nutr (Berl). 2021 Mar;105(2):247-259. doi: 10.1111/jpn.13470. Epub 2020 Nov 19.
The effect of Spirulina (Arthrospira platensis), individually or in combination with two commercial carbohydrases, in piglet diets was assessed on growth performance, nutrient digestibility and meat quality traits. Forty post-weaned male piglets from Large White × Landrace sows crossed with Pietrain boars with an initial live weight of 12.0 ± 0.89 kg were used. Piglets were assigned to one of four dietary treatments (n = 10): cereal and soya bean meal base diet (control), base diet with 10% Spirulina (SP), SP diet supplemented with 0.005% Rovabio Excel AP (SP + R) and SP diet supplemented with 0.01% lysozyme (SP + L). Animals were slaughtered after a 4-week experimental period. Growth performance was negatively affected by the incorporation of Spirulina in the diets, with an average decrease of 9.1% on final weight, in comparison with control animals. Total tract apparent digestibility (TTAD) of crude protein was higher (p < .05) in the control group than in other groups. In addition, lysozyme increased TTAD of crude fat and acid detergent fibre, relative to the SP and control groups, respectively. In addition, the incorporation of Spirulina, individually and supplemented with enzymes, did not impair meat quality traits. Surprisingly, no protective effect against lipid oxidation was observed with the inclusion of Spirulina in pork after 7 days of storage. This study indicates that growth performance of post-weaning piglets was impaired by the incorporation of 10% Spirulina in the diets, which is mediated by an increase in digesta viscosity and a lower protein digestibility, as a consequence of the resistance of microalga proteins to the action of endogenous peptidases. In addition, it also indicates that lysozyme, in contrast to Rovabio Excel AP, is efficient in the degradation of Spirulina cell wall in piglet's intestine. However, the digestion of proteins liberated by Spirulina cell wall disruption is still a challenge.
评估了螺旋藻（Arthrospira platensis）单独或与两种商业碳水化合物酶组合在仔猪日粮中的效果，对生长性能、养分消化率和肉质特性进行了评估。使用来自长白猪×兰德瑞斯母猪与皮特兰公猪杂交的 40 头断奶后雄性仔猪，初始活重为 12.0±0.89kg。仔猪被分配到四种日粮处理之一（n=10）：谷物和豆粕基础日粮（对照）、基础日粮添加 10%螺旋藻（SP）、SP 日粮添加 0.005% Rovabio Excel AP（SP+R）和 SP 日粮添加 0.01%溶菌酶（SP+L）。动物在 4 周实验期后被屠宰。生长性能受到日粮中螺旋藻添加的负面影响，与对照组相比，最终体重平均下降 9.1%。与对照组相比，粗蛋白的全肠道表观消化率（TTAD）在对照组中更高（p<.05）。此外，与 SP 和对照组相比，溶菌酶分别提高了粗脂肪和酸性洗涤剂纤维的 TTAD。此外，单独添加螺旋藻和添加酶均未损害肉质特性。令人惊讶的是，在猪肉中添加螺旋藻后 7 天的储存期内，没有观察到对脂质氧化的保护作用。本研究表明，在日粮中添加 10%螺旋藻会损害断奶仔猪的生长性能，这是由于微藻蛋白对内源性肽酶的抵抗力增加了食糜的粘性和降低了蛋白质的消化率。此外，这也表明溶菌酶与 Rovabio Excel AP 不同，能够有效地降解仔猪肠道中的螺旋藻细胞壁。然而，螺旋藻细胞壁破坏释放的蛋白质的消化仍然是一个挑战。
