Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China,Department of Cardiology, The Fourth Hospital ofChangsha, Changsha, Hunan, China,Department of Spine Surgery, Longhui People's Hospital, Shaoyang, Hunan, China,Department of Orthopedics, The FirstPeople's Hospital of Changde City, Changde, Hunan, China,Department of Emergency, The First Hospital of Changsha City, Changsha, Hunan, China,Departmentof Orthopedics, Xiangtan Central Hospital, Xiangtan, Hunan, China.
Medicine (Baltimore). 2022 Mar 18;101(11). doi: 10.1097/MD.0000000000028933.
Previous studies indicated that lamin proteins were thought to be related to gene expression, chromatin structure, and unclear stability. There are 2 types of vertebrate lamins, including A and B. The 2 B type proteins are encoded by lamin B1 (LMNB1) and lamin B2 (LMNB2). The LMNBs factor has been found to be associated with the development of multiple tumors, but its association with sarcoma has been barely mentioned.The transcription levels of LMNBs were analyzed via Oncomine database. Gene Expression Profiling Interactive Analysis (GEPIA) dataset was adopted to analyze the differential expression of LMNBs in sarcoma. Cancer Cell Line Encyclopedia dataset was used to explore the expression of LMNBs in sarcoma cell line. We analyzed the prognostic value of LMNBs in GEPIA and Kaplan-Meier Plotter. Oncomine and GEPIA datasets were also used to detect the relationship between LMNBs and their co-expressed genes. We used the Database for Annotation, Visualization and Integrated Discovery to conduct the Gene Ontology analysis of LMNBs and their co-expressed genes. Kyoto Encyclopedia of Genes and Genomes was also used to analyze the pathway of LMNBs.LMNB1 and LMNB2 were reported to be hyperexpressed in sarcoma. The expression of LMNBs was elevated in various sarcoma cell lines. According to the results, we observed that LMNBs were connected to the poor overall survival, recurrence-free survival, and disease-free survival of sarcoma patients.This study indicated that hyperexpression of LMNBs was significantly related to worse outcome of sarcoma, LMNB1 and LMNB2 were expected to become potential biomarkers for human.
先前的研究表明，核纤层蛋白与基因表达、染色质结构和稳定性有关。脊椎动物中有 2 种 lamin 蛋白，包括 A 型和 B 型。其中 2 种 B 型蛋白由 lamin B1（LMNB1）和 lamin B2（LMNB2）编码。现已发现 LMNBs 因子与多种肿瘤的发生发展有关，但与肉瘤的关系鲜有提及。通过 Oncomine 数据库分析 LMNBs 的转录水平。采用基因表达谱分析交互分析（GEPIA）数据集分析 LMNBs 在肉瘤中的差异表达。使用癌症细胞系百科全书数据集探索 LMNBs 在肉瘤细胞系中的表达。我们在 GEPIA 和 Kaplan-Meier Plotter 中分析了 LMNBs 的预后价值。还使用 Oncomine 和 GEPIA 数据集来检测 LMNBs 与其共表达基因之间的关系。我们使用数据库进行注释、可视化和综合发现对 LMNBs 及其共表达基因进行基因本体论分析。京都基因与基因组百科全书还用于分析 LMNBs 的通路。有报道称 LMNB1 和 LMNB2 在肉瘤中过表达。在各种肉瘤细胞系中，LMNBs 的表达上调。结果表明，我们观察到 LMNBs 与肉瘤患者的总体生存率、无复发生存率和无病生存率较差有关。这项研究表明，LMNBs 的高表达与肉瘤的预后不良显著相关，LMNB1 和 LMNB2 有望成为人类潜在的生物标志物。
