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利用洋葱内生细菌生物合成银纳米颗粒及其对水稻病原菌的抗真菌活性

Biosynthesis of Silver Nanoparticles Using Onion Endophytic Bacterium and Its Antifungal Activity against Rice Pathogen .

作者信息

Ibrahim Ezzeldin, Luo Jinyan, Ahmed Temoor, Wu Wenge, Yan Chenqi, Li Bin

机构信息

State Key Laboratory of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.

Department of Vegetable Diseases Research, Plant Pathology Research Institute, Agriculture Research Centre, Giza 12916, Egypt.

出版信息

J Fungi (Basel). 2020 Nov 18;6(4):294. doi: 10.3390/jof6040294.

DOI:10.3390/jof6040294
PMID:33217899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7712207/
Abstract

Biosynthesis of silver nanoparticles (AgNPs) using endophytic bacteria is a safe alternative to the traditional chemical method. The purpose of this research is to biosynthesize AgNPs using endophytic bacterium strain H3 isolated from onion. The biosynthesized AgNPs with sizes from 4.17 to 26.9 nm were confirmed and characterized by various physicochemical techniques such as Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), UV-visible spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) in addition to an energy dispersive spectrum (EDS) profile. The biosynthesized AgNPs at a concentration of 40 μg/mL had a strong antifungal activity against rice blast pathogen with an inhibition rate of 88% in mycelial diameter. Moreover, the biosynthesized AgNPs significantly inhibited spore germination and appressorium formation of . Additionally, microscopic observation showed that mycelia morphology was swollen and abnormal when dealing with AgNPs. Overall, the current study revealed that AgNPs could protect rice plants against fungal infections.

摘要

利用内生细菌生物合成银纳米颗粒(AgNPs)是传统化学方法的一种安全替代方案。本研究的目的是利用从洋葱中分离出的内生细菌菌株H3生物合成AgNPs。通过傅里叶变换红外光谱(FT-IR)、X射线衍射(XRD)、紫外可见光谱、透射电子显微镜(TEM)和扫描电子显微镜(SEM)等多种物理化学技术,以及能量色散谱(EDS)图谱,对生物合成的尺寸在4.17至26.9纳米之间的AgNPs进行了确认和表征。浓度为40μg/mL的生物合成AgNPs对稻瘟病菌具有很强的抗真菌活性,菌丝直径抑制率达88%。此外,生物合成的AgNPs显著抑制了稻瘟病菌的孢子萌发和附着胞形成。另外,显微镜观察表明,处理AgNPs时菌丝形态肿胀且异常。总体而言,当前研究表明AgNPs可以保护水稻植株免受真菌感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/9ea666399ce9/jof-06-00294-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/f5b1949c9d20/jof-06-00294-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/85045585e138/jof-06-00294-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/349379fcabf7/jof-06-00294-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/5974d52b2060/jof-06-00294-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/c0a82702d69b/jof-06-00294-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/79ce8f413de4/jof-06-00294-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/5cdd00f43c40/jof-06-00294-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/97b8355ad0e9/jof-06-00294-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/9ea666399ce9/jof-06-00294-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/f5b1949c9d20/jof-06-00294-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/85045585e138/jof-06-00294-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/349379fcabf7/jof-06-00294-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/5974d52b2060/jof-06-00294-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/c0a82702d69b/jof-06-00294-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/79ce8f413de4/jof-06-00294-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/5cdd00f43c40/jof-06-00294-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/97b8355ad0e9/jof-06-00294-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db0/7712207/9ea666399ce9/jof-06-00294-g009.jpg

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