低分化 CD34+ 细胞中纤维细胞和内皮祖细胞在肺结节病进展中的作用。

Role of fibrocytes and endothelial progenitor cells among low-differentiated CD34+ cells in the progression of lung sarcoidosis.

机构信息

Department of Internal Medicine and Hematology, Laboratory of Hematology and Flow Cytometry, Military Institute of Medicine, Warsaw, Poland.

Department of Internal Medicine, Pulmonology, Allergology and Clinical Immunology, Military Institute of Medicine, Warsaw, Poland.

出版信息

BMC Pulm Med. 2020 Nov 20;20(1):306. doi: 10.1186/s12890-020-01345-x.

DOI:10.1186/s12890-020-01345-x

PMID:33218322

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7678043/

Abstract

BACKGROUND

Sarcoidosis is a multisystemic granulomatous disease with still unknown etiology. Our previous studies showed a significantly higher percentage of CD34 + cells in the peripheral blood in patients with sarcoidosis (SA) compared to the control group. The objective of the present study was to characterized of the CD34 + cell population in peripheral blood in patients with SA with reference to the control group. Moreover in patients with SA, fibrocytes and endothelial cells were analysed and their relationship to the fibrosis process based on assessment of diffusing capacity for carbon monoxide (DLCO).

METHODS

Data from patients diagnosed with SA at Military Institute of Medicine (Warsaw, Poland) between January 2018 and December 2019 were collected and analysed ongoing basis. Peripheral blood was collected from 26 patients with newly diagnosed pulmonary SA and 16 healthy subjects. The immunomagnetic method and flow cytometry were used. Among the CD34+ progenitor cells were assessed: low-differentiated cells, hematopoietic progenitor cells and endothelial progenitor cells. The Statistica 12.0 software was used for a statistical analysis.

RESULTS

We observed a significantly higher percentage of low-differentiated cells (13.8 vs. 2.3, P = 0.001) and endothelial cells (0.3 vs. 0.0, P = 0.001) in patients with SA compared to the control group. In the study group the median proportion of fibrocytes was 1.877% (0.983-2.340) in patients with DLCO< 80%, while in patients with DLCO> 80% was 0.795% (0.139-1.951) (P = 0.72). The median proportion of endothelial progenitor cells was higher in patients with DLCO< 80%: 0.889% (0.391-1.741), than in patients with DLCO> 80%: 0.451% (0.177-0.857) (P = 0.44).

CONCLUSIONS

In conclusion we demonstrated for the first time the immunophenotype of peripheral CD34 + cells with the degree of their differentiation. The study confirmed the involvement of low differentiated cells and endothelial cells in patients with SA.

摘要

背景

结节病是一种多系统肉芽肿性疾病，其病因仍不清楚。我们之前的研究表明，与对照组相比，结节病（SA）患者外周血中 CD34+细胞的百分比明显更高。本研究的目的是参照对照组，对 SA 患者外周血中 CD34+细胞群进行特征描述。此外，在 SA 患者中，还分析了纤维母细胞和内皮细胞，并基于一氧化碳弥散量（DLCO）评估，分析了它们与纤维化过程的关系。

方法

收集了 2018 年 1 月至 2019 年 12 月期间在波兰军事医学研究所（华沙）诊断为 SA 的患者的数据，并持续进行分析。从 26 例新诊断的肺 SA 患者和 16 例健康受试者中采集外周血。使用免疫磁珠法和流式细胞术进行检测。在 CD34+祖细胞中评估了低分化细胞、造血祖细胞和内皮祖细胞。使用 Statistica 12.0 软件进行统计分析。

结果

与对照组相比，SA 患者的低分化细胞（13.8%比 2.3%，P=0.001）和内皮细胞（0.3%比 0.0%，P=0.001）的百分比明显更高。在研究组中，DLCO<80%的患者纤维母细胞中位数比例为 1.877%（0.983-2.340），而 DLCO>80%的患者为 0.795%（0.139-1.951）（P=0.72）。DLCO<80%的患者中内皮祖细胞的中位数比例较高，为 0.889%（0.391-1.741），而 DLCO>80%的患者为 0.451%（0.177-0.857）（P=0.44）。