Feik S A, Storey E, Ellender G
Department of Preventive and Community Dentistry, University of Melbourne, Victoria, Australia.
Br J Exp Pathol. 1987 Dec;68(6):803-13.
The tails of Sprague-Dawley rats of approximately 50 g body mass were either left straight or bent to form a loop containing three or five vertebrae (CV). Subsequent treatment was as follows: (a) in situ: segments were removed at 0, 8, 16, 24 h, 3, 5 and 7 days and examined histologically: (b) transplants: skinned segments were transplanted autologously and examined at the same time intervals; (c) normal saline: skinned segments were placed in a 0.9% solution maintained at (i) 4 degrees C and (ii) 37 degrees C and examined 8, 16 and 24 h later. The results show that on bending a bone in situ the remodelling which occurs reflects the displacement of the soft tissues, and more specifically the periosteum, towards or away from the bone surface. Functionally the developing periosteum consists of three zones and when stressed its fibroelastic component moves away from or towards the osteogenic layer either applying tension to it stimulating bone formation, or pressure eventually inducing bone resorption. These changes are mediated via the mid-zone. The effects on the fibroelastic component alone are best seen in transplants or in normal saline at 37 degrees C where the osteogenic layer dies. The periosteum in growing bones is ideally structured to respond rapidly and sensitively to altered strain by initiating adaptive surface remodelling of bone.
体重约50克的斯普拉格-道利大鼠的尾巴要么保持伸直，要么弯曲形成一个包含三或五个椎骨（CV）的环。后续处理如下：（a）原位：在0、8、16、24小时、3、5和7天取出节段并进行组织学检查；（b）移植：将去皮节段进行自体移植，并在相同时间间隔进行检查；（c）生理盐水：将去皮节段置于0.9%的溶液中，分别在（i）4℃和（ii）37℃下保存，并在8、16和24小时后进行检查。结果表明，原位弯曲骨骼时发生的重塑反映了软组织，更具体地说是骨膜，朝向或远离骨表面的位移。从功能上讲，发育中的骨膜由三个区域组成，当受到应力时，其纤维弹性成分会远离或朝向成骨层移动，要么对其施加张力刺激骨形成，要么施加压力最终诱导骨吸收。这些变化是通过中间区域介导的。单独对纤维弹性成分的影响在移植或37℃的生理盐水中最为明显，此时成骨层会死亡。生长中的骨骼中的骨膜结构理想，能够通过启动骨的适应性表面重塑来快速而敏感地响应应变变化。
