INRAE, QuaPA, 63122 Saint-Gènes-Champanelle, France.
Molecules. 2020 Nov 19;25(22):5422. doi: 10.3390/molecules25225422.
Collagen antioxidant peptides are being widely studied. However, no research has paid attention to biological parameters such as the age and anatomy of collagen-rich tissues, which can determine a change in tissue structure and composition, and then in bioactivity. Moreover, only few research works have studied and assessed peptides antioxidant activity on the food matrix. This work aimed to investigate the effect of bovine's bone age and anatomy, and of six different enzymes, on the antioxidant activity of collagen peptides. Collagen was extracted from young and old bovine femur and tibia; six different enzymes were used for peptides' release. The redox potential, the quenching of stable free radicals, and the antioxidant capacity on bovine meat lipids and proteins was evaluated, under heating from ambient temperature to 80 °C. Age and anatomy showed a significant effect; the influence of anatomy becomes most important with age. Each enzyme's effectiveness toward age and anatomy was not the same. The greatest amount of peptides was released from young bones' collagen hydrolysed with papain. The antioxidant activity was higher at higher temperatures, except for meat proteins. Assessing the effect of age and anatomy of collagen-rich tissues can promote a better application of collagen bioactive peptides.
胶原蛋白抗氧化肽正在被广泛研究。然而，目前还没有研究关注胶原蛋白丰富的组织的年龄和解剖结构等生物学参数，这些参数可以决定组织结构和组成的变化，进而影响生物活性。此外，只有少数研究工作研究并评估了肽在食品基质上的抗氧化活性。本工作旨在研究牛骨年龄和解剖结构以及六种不同酶对胶原蛋白肽抗氧化活性的影响。从年轻和年老的牛股骨和胫骨中提取胶原蛋白；使用六种不同的酶释放肽。在从环境温度加热到 80°C 的过程中，评估了还原电势、稳定自由基的淬灭以及对牛肉脂质和蛋白质的抗氧化能力。年龄和解剖结构有显著影响；随着年龄的增长，解剖结构的影响变得越来越重要。木瓜蛋白酶水解年轻骨骼胶原蛋白释放的肽最多。除了肉类蛋白质外，抗氧化活性在较高温度下更高。评估富含胶原蛋白的组织的年龄和解剖结构的影响可以促进胶原蛋白生物活性肽的更好应用。
