State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 510060, China.
Graefes Arch Clin Exp Ophthalmol. 2021 Apr;259(4):929-940. doi: 10.1007/s00417-020-05017-8. Epub 2020 Nov 25.
To investigate the therapeutic effect of subconjunctival injection of tumor necrosis factor-α (TNF-α) pre-stimulated bone marrow-derived mesenchymal stem cells (BMMSCs) on ocular alkali burns in a rat model.
After applying a 6 mm filter paper soaking in 1 N NaOH on the cornea of rats, the suspension of TNF-α pre-stimulated BMMSCs, BMMSCs and PBS were given subconjunctivally and respectively. Corneal epithelial defect, corneal opacity, inflammation as well as PTGS2 and TSG-6 expression on day 7 and fibrosis on day 14 were compared.
TNF-α pre-stimulated BMMSCs group had a more predominate effect on promoting corneal epithelial repairing, decreasing corneal opacity, reducing inflammatory cells and CD68 + macrophages on day 7 and suppressing fibrosis on day 14 compared to BMMSCs group. Besides, it had significant increased expressions of PTGS2 and TSG-6 in vitro. Pre-treated with Indomethacin revealed a reverse effect on above-mentioned changes.
Subconjunctival injection of TNF-α pre-stimulated BMMSCs enhanced anti-inflammatory and anti-fibrotic effect in ocular alkali burns, which was possibly though up regulation of PTGS2 and TSG-6 expression.
观察 TNF-α 预处理骨髓间充质干细胞（BMMSCs）经结膜下注射对大鼠眼碱烧伤的治疗作用。
采用 6mm 滤纸片蘸 1NNaOH 溶液贴敷角膜造成大鼠眼碱烧伤模型，于伤后即刻分别经结膜下注射 TNF-α 预处理 BMMSCs、BMMSCs 悬液及 PBS。于第 7 天观察角膜上皮缺损、混浊度、炎症反应，第 14 天观察角膜纤维化程度，并检测 PTGS2 和 TSG-6 表达。
与 BMMSCs 组相比，TNF-α 预处理 BMMSCs 组在促进角膜上皮修复、减轻角膜混浊、减少炎症细胞和 CD68+巨噬细胞浸润以及抑制角膜纤维化方面作用更为明显，同时 PTGS2 和 TSG-6 的表达也明显上调。预先给予非甾体类抗炎药吲哚美辛可逆转上述变化。
TNF-α 预处理 BMMSCs 经结膜下注射可增强眼碱烧伤的抗炎和抗纤维化作用，其机制可能与上调 PTGS2 和 TSG-6 的表达有关。
