Immunomodulatory Function of Polyvinylpyrrolidone (PVP)-Functionalized Gold Nanoparticles in -Stimulated Sea Urchin Immune Cells.

We investigated the role of the gold nanoparticles functionalized with polyvinylpyrrolidone (PVP-AuNPs) on the innate immune response against an acute infection caused by in an in vitro immunological nonmammalian next-generation model, the sea urchin . To profile the immunomodulatory function of PVP-AuNPs (0.1 μg mL) in sea urchin immune cells stimulated by (10 μg mL) for 3 h, we focused on the baseline immunological state of the donor, and we analysed the topography, cellular metabolism, and expression of human cell surface antigens of the exposed cells, as well as the signalling leading the interaction between PVP-AuNPs and the -stimulated cells. PVP-AuNPs are not able to silence the inflammatory signalling (TLR4/p38MAPK/NF-κB signalling) that involves the whole population of immune cells exposed to . However, our findings emphasise the ability of PVP-AuNPs to stimulate a subset of rare cells (defined here as Group 3) that express CD45 and CD14 antigens on their surface, which are known to be involved in immune cell maturation and macrophage activation in humans. Our evidence on how PVP-AuNPs may stimulate sea urchin immune cells represents an important starting point for planning new research work on the topic.