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功能性蛋白激酶C参与对H-ras癌基因产物的促有丝分裂反应。

Involvement of functional protein kinase C in the mitogenic response to the H-ras oncogene product.

作者信息

Lacal J C, Fleming T P, Warren B S, Blumberg P M, Aaronson S A

机构信息

Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.

出版信息

Mol Cell Biol. 1987 Nov;7(11):4146-9. doi: 10.1128/mcb.7.11.4146-4149.1987.

DOI:10.1128/mcb.7.11.4146-4149.1987
PMID:3323889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368090/
Abstract

Microinjection of purified protein kinase C (PKC) into Swiss 3T3 fibroblasts pretreated with the phorbol ester phorbol-12,13-dibutyrate restores the mitogenic response of the cells to phorbol-12,13-dibutyrate (G. Pasti, J.C. Lacal, B.S. Warren, S.A. Aaronson, and P.M. Blumberg, Nature [London] 324:375-377, 1986). Our present studies demonstrate that the mitogenic activity of the H-ras oncogene in H-ras p21-microinjected quiescent cells is markedly reduced under conditions in which PKC is downregulated by chronic phorbol ester treatment. The ability to reconstitute the mitogenic response upon microinjection of both H-ras p21 and PKC implies involvement of functional PKC in the mitogenic activity of the H-ras oncogene product.

摘要

将纯化的蛋白激酶C（PKC）显微注射到用佛波酯佛波醇-12,13-二丁酸预处理过的瑞士3T3成纤维细胞中，可恢复细胞对佛波醇-12,13-二丁酸的促有丝分裂反应（G. 帕斯蒂、J.C. 拉卡尔、B.S. 沃伦、S.A. 阿伦森和P.M. 布隆伯格，《自然》[伦敦] 324:375 - 377，1986年）。我们目前的研究表明，在通过长期佛波酯处理使PKC下调的条件下，显微注射H-ras p21的静止细胞中H-ras癌基因的促有丝分裂活性显著降低。显微注射H-ras p21和PKC后重建促有丝分裂反应的能力意味着功能性PKC参与了H-ras癌基因产物的促有丝分裂活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d99/368090/e60a6dcd95b1/molcellb00083-0293-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d99/368090/b6d1cb1efcd8/molcellb00083-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d99/368090/e60a6dcd95b1/molcellb00083-0293-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d99/368090/b6d1cb1efcd8/molcellb00083-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d99/368090/e60a6dcd95b1/molcellb00083-0293-b.jpg

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本文引用的文献

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Transformation of NIH 3T3 cells by microinjection of Ha-ras p21 protein.通过显微注射Ha-ras p21蛋白对NIH 3T3细胞进行转化。
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Microinjection of the oncogene form of the human H-ras (T-24) protein results in rapid proliferation of quiescent cells.显微注射人H-ras（T-24）蛋白的致癌基因形式会导致静止细胞快速增殖。
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Human oncogenes.人类癌基因。
致癌性Ha-Ras在小鼠乳腺上皮细胞中对c-fos的转录激活需要蛋白激酶C-λ、ε和ζ的联合活性。
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p21ras and protein kinase C function in distinct and interdependent signaling pathways in C3H 10T1/2 fibroblasts.p21ras和蛋白激酶C在C3H 10T1/2成纤维细胞中不同且相互依赖的信号通路中发挥作用。
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Nature. 1986;324(6095):375-7. doi: 10.1038/324375a0.
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