Jin Feng, Zhou Zhaoli, Guo Qi, Liang Zhenwen, Yang Ruoyu, Jiang Jibao, He Yanlin, Zhao Qi, Zhao Qiang
College of Rehabilitation Sciences, Shanghai University of Medicine and Health Sciences, Shanghai, China.
Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai, China.
PeerJ. 2020 Nov 12;8:e10313. doi: 10.7717/peerj.10313. eCollection 2020.
Earthworms enrich the soil and protect the health of their ecological environment. Previous studies on these invertebrates determined their protein content, hormone secretions, medicinal value, and ecological habits, but their whole genomic sequence remains incomplete. We performed whole genome sequencing of (Chen, 1930), which belongs to the genus of the family Megascolecidae. The genome assembly was 729 Mb, with a N50 contig size of 4.2 Mb. In total, 559 contigs were anchored to 41 chromosomes according to the results of Hi-C (High-throughput Chromosome Conformation Capture) technology, which was confirmed by karyological analysis. A comparison of the genomic sequences and genes indicated that there was a whole-genome duplication in followed by several chromosome fusion events. Hox genes and lumbrokinase genes were identified as partial clusters surrounding the genome. Our high-quality genome assembly of will provide valuable information for gene function and evolutionary studies in earthworms.
蚯蚓能使土壤肥沃并保护其生态环境的健康。此前对这些无脊椎动物的研究确定了它们的蛋白质含量、激素分泌、药用价值和生态习性，但它们的全基因组序列仍不完整。我们对巨蚓科环毛蚓属的参环毛蚓（陈义，1930）进行了全基因组测序。基因组组装大小为729 Mb，N50重叠群大小为4.2 Mb。根据高通量染色体构象捕获（Hi-C）技术的结果，共有559个重叠群被锚定到41条染色体上，这一点通过核型分析得到了证实。基因组序列和基因的比较表明，参环毛蚓经历了一次全基因组复制，随后发生了几次染色体融合事件。Hox基因和蚓激酶基因被确定为基因组周围的部分基因簇。我们高质量的参环毛蚓基因组组装将为蚯蚓的基因功能和进化研究提供有价值的信息。
