College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611134, China.
Nutrients. 2022 Feb 18;14(4):857. doi: 10.3390/nu14040857.
L. (PE) is commonly known as a medicine and food homologous plant, which is abundant in natural products polyphenols. In the present study, polyphenols were extracted from PE fruit by response surface method, and the anti-aging ability was determined. PE fruit polyphenols exhibited strong antioxidant capacities in scavenging free radicals, and anti-cholinesterase ability by inhibition of AChE (IC 0.2186 ± 0.0416 mg/mL) and BuChE (IC 0.0542 ± 0.0054 mg/mL) in vitro. Moreover, PE fruit polyphenols showed strong protective effect against the aging process in model, including increased thermal resistance, extended lifespan by 18.53% ( < 0.05), reduced activity of AChE by 34.71% and BuChE by 45.38% ( < 0.01). This was accompanied by the enhancement in antioxidant enzymes activity of SOD by 30.74% ( < 0.05) and CAT by 8.42% ( > 0.05), while decrease in MDA level by 36.25% ( < 0.05). These properties might be interrelated with the presence of abundant flavonols and phenolic acids identified by UPLC-ESI-QTOF-MS, such as quercetin, myricetin, ellagic, gallic, and chlorogenic acids, together with their glycosides. The remarkable antioxidant and anti-aging potential of PE fruit polyphenols could be implemented in the food and pharmaceutical industry.
L.（PE）通常被称为药食同源植物，其天然产物富含多酚。本研究采用响应面法从 PE 果实中提取多酚，并测定其抗衰老能力。PE 果实多酚具有较强的清除自由基和抑制 AChE（IC 0.2186 ± 0.0416 mg/mL）和 BuChE（IC 0.0542 ± 0.0054 mg/mL）的体外抗氧化能力。此外，PE 果实多酚对模型中的衰老过程具有较强的保护作用，包括提高耐热性、寿命延长 18.53%（<0.05）、AChE 和 BuChE 活性分别降低 34.71%和 45.38%（<0.01）。这伴随着 SOD 抗氧化酶活性提高 30.74%（<0.05）和 CAT 提高 8.42%（>0.05），同时 MDA 水平降低 36.25%（<0.05）。这些特性可能与通过 UPLC-ESI-QTOF-MS 鉴定的丰富的类黄酮和酚酸有关，如槲皮素、杨梅素、鞣花酸、没食子酸和绿原酸及其糖苷。PE 果实多酚具有显著的抗氧化和抗衰老潜力，可应用于食品和制药工业。
