The State Key Lab of Pharmaceutical Biotechnology, College of life Sciences, Nanjing University, Nanjing 210023, China.
Molecules. 2020 Nov 25;25(23):5523. doi: 10.3390/molecules25235523.
()-induced acute lung injury (ALI) is a serious disease that has a high risk of death among infants and teenagers. Acetylharpagide, a natural compound of Thunb. (family Labiatae), has been found to have anti-tumor, anti-inflammatory and anti-viral effects. This study investigates the therapeutic effects of acetylharpagide on -induced ALI in mice. Here, we found that acetylharpagide alleviated -induced lung pathological morphology damage, protected the pulmonary blood-gas barrier and improved the survival of -infected mice. Furthermore, -induced myeloperoxidase (MPO) activity of lung homogenate and pro-inflammatory factors in bronchoalveolar lavage (BAL) fluid were suppressed by acetylharpagide. Mechanically, acetylharpagide inhibited the interaction between polyubiquitinated receptor interacting protein 1 (RIP1) and NF-κB essential modulator (NEMO), thereby suppressing NF-κB activity. In summary, these results show that acetylharpagide protects mice from -induced ALI by suppressing the NF-κB signaling pathway. Acetylharpagide is expected to become a potential treatment for -induced ALI.
（）诱导的急性肺损伤（ALI）是一种严重的疾病，婴儿和青少年的死亡率很高。已发现乙酰哈巴苷是（唇形科）的一种天然化合物，具有抗肿瘤、抗炎和抗病毒作用。本研究探讨了乙酰哈巴苷对（）诱导的小鼠 ALI 的治疗作用。在这里，我们发现乙酰哈巴苷减轻了（）诱导的肺病理形态损伤，保护了肺气血屏障，并提高了感染（）的小鼠的存活率。此外，乙酰哈巴苷抑制了肺匀浆中髓过氧化物酶（MPO）活性和支气管肺泡灌洗液（BAL）中促炎因子的产生。在机制上，乙酰哈巴苷抑制了多聚泛素化受体相互作用蛋白 1（RIP1）和 NF-κB 必需调节剂（NEMO）之间的相互作用，从而抑制了 NF-κB 活性。总之，这些结果表明，乙酰哈巴苷通过抑制 NF-κB 信号通路来保护小鼠免受（）诱导的 ALI。乙酰哈巴苷有望成为（）诱导的 ALI 的一种潜在治疗方法。
